LSBio
PRAMEF20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PRAMEF20 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PRAMEF20 antibodies →
$725.00each
Catalog Number: LS-G119172-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRAMEF20 (NM_001099852), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001099852

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MSIRTPPRLL ELAGRSLLRD EALAISTLEE LPTELFPPLF MEAFSRRHCE ALKLMVQAWP
61FLRLPLGSLM KRPCPETFQA VLDGLDALLT HRVRLRRWKL QVLDLQDVSE NFWMVWSEAM
121ARRCLPNAMM NRKPVQDCPR MRGQQPLTVF IDLCLKNRTL DEYFTCLFLW VKQREGLVHL
181CCKKLKMLGM LFHNIRNILK TVNLDCIQEV EVNCNWTLPV LAEFTPYLGQ MRNLRKLVLS
241DIDSRYISPE QKKEFVTQFT TQFLKLRCLQ KLYMNSVSFL EGHLDQMLSC LKTSLNILAI
301TNCVLLESDL KHLSKYPSIG QLKTLDLSGT RLANFSLVPL QVLLEKVAAT LEYLDLDDCG
361IVDSQVNAIL PALSRCFELT TFSFRGNPIS TATLENLLCH TIRLNNLCLE LYPAPRESYD
421VRGIVCRSRF AQLGAELMGR VRALREPERI LFCTDYCPQC GNRSLYDLEV DRCCC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

475 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.