PRAMEF20 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G119172-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRAMEF20 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95659-20
Catalog Number: LS-G95659
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRAMEF21 (NM_001100114) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001099852
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSIRTPPRLL ELAGRSLLRD EALAISTLEE LPTELFPPLF MEAFSRRHCE ALKLMVQAWP61FLRLPLGSLM KRPCPETFQA VLDGLDALLT HRVRLRRWKL QVLDLQDVSE NFWMVWSEAM121ARRCLPNAMM NRKPVQDCPR MRGQQPLTVF IDLCLKNRTL DEYFTCLFLW VKQREGLVHL181CCKKLKMLGM LFHNIRNILK TVNLDCIQEV EVNCNWTLPV LAEFTPYLGQ MRNLRKLVLS241DIDSRYISPE QKKEFVTQFT TQFLKLRCLQ KLYMNSVSFL EGHLDQMLSC LKTSLNILAI301TNCVLLESDL KHLSKYPSIG QLKTLDLSGT RLANFSLVPL QVLLEKVAAT LEYLDLDDCG361IVDSQVNAIL PALSRCFELT TFSFRGNPIS TATLENLLCH TIRLNNLCLE LYPAPRESYD421VRGIVCRSRF AQLGAELMGR VRALREPERI LFCTDYCPQC GNRSLYDLEV DRCCCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
475 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
