Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRAMEF14 / PRAMEF13 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119171-100
Catalog Number: LS-G119171
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRAMEF14 (NM_001099854), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PRAME family member 13
- Origin Species
- Human
- NCBI GenBank
- NM_001024661
- SwissProt
- Q5SWL7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSIQAPPRLL ELAGQSLLRD QALSISAMEE LPRVLYLPLF MEAFRRRHFQ TLTVMVQAWP61FTCLPLGSLM KTLHLETLKA LLEGLHMLLT QKDRPRRWKL QVLDLRDVDE NFWARWPGAW121ALSCFPETMS KRQTAEDCPR MGEHQPLKVF IDICLKEIPQ DECLRYLFQW VYQRRGLVHL181CCSKLVNYLT PIKHLRKSLK IIYLNSIQQL EIRNMSWPRL IRKLRCYLKE MKNLRKLVFS241RCLQNPLENL ELTYGYLLEE DMKCLSQYPS LGYLKHLNLS YVLLFRISLE PLGALLEKIA301ASLETLILEG CQIHYSQLSA ILPGLSHCSQ LTTFYFGRNC MSMGALKDLL CHTSGLSKLS361LETYPAPEES LNSLVRVDWE IFALLRAELM CTLREVRQPK RIFIGPTPCP SCGSSPSEEL421ELHLCCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
426 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.