PRAMEF14 / PRAMEF13 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G119171-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRAMEF14 / PRAMEF13 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G96271-20
Catalog Number: LS-G96271
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRAMEF14 (NM_001024661) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PRAME family member 13
- Origin Species
- Human
- NCBI GenBank
- NM_001024661
- SwissProt
- Q5SWL7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSIQAPPRLL ELAGQSLLRD QALSISAMEE LPRVLYLPLF MEAFRRRHFQ TLTVMVQAWP61FTCLPLGSLM KTLHLETLKA LLEGLHMLLT QKDRPRRWKL QVLDLRDVDE NFWARWPGAW121ALSCFPETMS KRQTAEDCPR MGEHQPLKVF IDICLKEIPQ DECLRYLFQW VYQRRGLVHL181CCSKLVNYLT PIKHLRKSLK IIYLNSIQQL EIRNMSWPRL IRKLRCYLKE MKNLRKLVFS241RCHHSMSDNE LEGRLVTKFS SVFLRLEHLQ LLKIKLITFF SGHLEQLIRC LQNPLENLEL301TYGYLLEEDM KCLSQYPSLG YLKHLNLSYV LLFRISLEPL GALLEKIAAS LETLILEGCQ361IHYSQLSAIL PGLSHCSQLT TFYFGRNCMS MGALKDLLRH TSGLSKLSLE TYPAPEESLN421SLVRVDWEIF ALLRAELMCT LREVRQPKRI FIGPTPCPSC GSSPSEELEL HLCCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
474 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
