Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PRAGMIN / SGK223 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G123924-100
Catalog Number: LS-G123924
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRAGMIN(SGK223) (NM_001080826), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Homolog of rat pragma of Rnd2 | PRAGMIN | Sugen kinase 223 | SGK223 | Tyrosine-protein kinase SgK223
- Origin Species
- Human
- NCBI GenBank
- AF075060
- SwissProt
- Q86YV5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 149.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHQTLCLNPE SLKMSACSDF VEHIWKPGSC KNCFCLRSDH QLVAGPPQPR AGSLPPPPRL61PPRPENCRLE DEGVNSSPYS KPTIAVKPTM MSSEASDVWT EANLSAEVSQ VIWRRAPGKL121PLPKQEDAPV VYLGSFRGVQ KPAGPSTSPD GNSRCPPAYT MVGLHNLEPR GERNIAFHPV181SFPEEKAVHK EKPSFPYQDR PSTQESFRQK LAAFAGTTSG CHQGPGPLRE SLPSEDDSDQ241RCSPSGDSEG GEYCSILDCC PGSPVAKAAS QTAGSRGRHG GRDCSPTCWE QGKCSGPAEQ301EKRGPSFPKE CCSQGPTAHP SCLGPKKLSL TSEAAISSDG LSCGSGSGSG ASSPFVPHLE361SDYCSLMKEP APEKQQDPGC PGVTPSRCLG LTGEPQPPAH PREATQPEPI YAESTKRKKA421APVPSKSQAK IEHAAAAQGQ GQVCTGNAWA QKAASGWGRD SPDPTPQVSA TITVMAAHPE481EDHRTIYLSS PDSAVGVQWP RGPVSQNSEV GEEETSAGQG LSSRESHAHS ASESKPKERP541AIPPKLSKSS PVGSPVSPSA GGPPVSPLAD LSDGSSGGSS IGPQPPSQGP ADPAPSCRTN601GVAISDPSRC PQPAASSASE QRRPRFQAGT WSRQCRIEEE EEVEQELLSH SWGRETKNGP661TDHSNSTTWH RLHPTDGSSG QNSKVGTGMS KSASFAFEFP KDRSGIETFS PPPPPPKSRH721LLKMNKSSSD LEKVSQGSAE SLSPSFRGVH VSFTTGSTDS LASDSRTCSD GGPSSELAHS781PTNSGKKLFA PVPFPSGSTE DVSPSGPQQP PPLPQKKIVS RAASSPDGFF WTQGSPKPGT841ASPKLNLSHS ETNVHDESHF SYSLSPGNRH HPVFSSSDPL EKAFKGSGHW LPAAGLAGNR901GGCGSPGLQC KGAPSASSSQ LSVSSQASTG STQLQLHGLL SNISSKEGTY AKLGGLYTQS961LARLVAKCED LFMGGQKKEL HFNENNWSLF KLTCNKPCCD SGDAIYYCAT CSEDPGSTYA1021VKICKAPEPK TVSYCSPSVP VHFNIQQDCG HFVASVPSSM LSSPDAPKDP VPALPTHPPA1081QEQDCVVVIT REVPHQTASD FVRDSAASHQ AEPEAYERRV CFLLLQLCNG LEHLKEHGII1141HRDLCLENLL LVHCTLQAGP GPAPAPAPAP AAAAPPCSSA APPAGGTLSP AAGPASPEGP1201REKQLPRLII SNFLKAKQKP GGTPNLQQKK SQARLAPEIV SASQYRKFDE FQTGILIYEL1261LHQPNPFEVR AQLRERDYRQ EDLPPLPALS LYSPGLQQLA HLLLEADPIK RIRIGEAKRV1321LQCLLWGPRR ELVQQPGTSE EALCGTLHNW IDMKRALMMM KFAEKAVDRR RGVELEDWLC1381CQYLASAEPG ALLQSLKLLQ LLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1402 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.