PRAGMIN / SGK223 Antibody
Catalog Number: LS-C809852
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PRAGMIN / SGK223 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83230-20
Catalog Number: LS-G83230
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PRAGMIN(SGK223) (NM_001080826) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Homolog of rat pragma of Rnd2 | PRAGMIN | Sugen kinase 223 | SGK223 | Tyrosine-protein kinase SgK223
- Origin Species
- Human
- NCBI GenBank
- AF075060
- SwissProt
- Q86YV5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 149.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHQTLCLNPE SLKMSACSDF VEHIWKPGSC KNCFCLRSDH QLVAGPPQPR AGSLPPPPRL61PPRPENCRLE DEGVNSSPYS KPTIAVKPTM MSSEASDVWT EANLSAEVSQ VIWRRAPGKL121PLPKQEDAPV VYLGSFRGVQ KPAGPSTSPD GNSRCPPAYT MVGLHNLEPR GERNIAFHPV181SFPEEKAVHK EKPSFPYQDR PSTQESFRQK LAAFAGTTSG CHQGPGPLRE SLPSEDDSDQ241RCSPSGDSEG GEYCSILDCC PGSPVAKAAS QTAGSRGRHG GRDCSPTCWE QGKCSGPAEQ301EKRGPSFPKE CCSQGPTAHP SCLGPKKLSL TSEAAISSDG LSCGSGSGSG ASSPFVPHLE361SDYCSLMKEP APEKQQDPGC PGVTPSRCLG LTGEPQPPAH PREATQPEPI YAESTKRKKA421APVPSKSQAK IEHAAAAQGQ GQVCTGNAWA QKAASGWGRD SPDPTPQVSA TITVMAAHPE481EDHRTIYLSS PDSAVGVQWP RGPVSQNSEV GEEETSAGQG LSSRESHAHS ASESKPKERP541AIPPKLSKSS PVGSPVSPSA GGPPVSPLAD LSDGSSGGSS IGPQPPSQGP ADPAPSCRTN601GVAISDPSRC PQPAASSASE QRRPRFQAGT WSRQCRIEEE EEVEQELLSH SWGRETKNGP661TDHSNSTTWH RLHPTDGSSG QNSKVGTGMS KSASFAFEFP KDRSGIETFS PPPPPPKSRH721LLKMNKSSSD LEKVSQGSAE SLSPSFRGVH VSFTTGSTDS LASDSRTCSD GGPSSELAHS781PTNSGKKLFA PVPFPSGSTE DVSPSGPQQP PPLPQKKIVS RAASSPDGFF WTQGSPKPGT841ASPKLNLSHS ETNVHDESHF SYSLSPGNRH HPVFSSSDPL EKAFKGSGHW LPAAGLAGNR901GGCGSPGLQC KGAPSASSSQ LSVSSQASTG STQLQLHGLL SNISSKEGTY AKLGGLYTQS961LARLVAKCED LFMGGQKKEL HFNENNWSLF KLTCNKPCCD SGDAIYYCAT CSEDPGSTYA1021VKICKAPEPK TVSYCSPSVP VHFNIQQDCG HFVASVPSSM LSSPDAPKDP VPALPTHPPA1081QEQDCVVVIT REVPHQTASD FVRDSAASHQ AEPEAYERRV CFLLLQLCNG LEHLKEHGII1141HRDLCLENLL LVHCTLQAGP GPAPAPAPAP AAAAPPCSSA APPAGGTLSP AAGPASPEGP1201REKQLPRLII SNFLKAKQKP GGTPNLQQKK SQARLAPEIV SASQYRKFDE FQTGILIYEL1261LHQPNPFEVR AQLRERDYRQ EDLPPLPALS LYSPGLQQLA HLLLEADPIK RIRIGEAKRV1321LQCLLWGPRR ELVQQPGTSE EALCGTLHNW IDMKRALMMM KFAEKAVDRR RGVELEDWLC1381CQYLASAEPG ALLQSLKLLQ LLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1402 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
