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PPP4R4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP4R4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPP4R4 antibodies →
$504.00each
Catalog Number: LS-G107946-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PP4R4(PPP4R4) (NM_058237), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PPP4R4 | PP4R4 | KIAA1622
Origin Species
Human
NCBI GenBank
NM_020958
SwissProt
Q6NUP7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHPPPPAAAM DFSQNSLFGY MEDLQELTII ERPVRRSLKT PEEIERLTVD EDLSDIERAV
61YLLSAGQDVQ GTSVIANLPF LMRQNPTETL RRVLPKVREA LHVAGVEMQL TAAMSFLTIL
121QDESVSIHAY THSFLQVILL HLEHRDTGVS NAWLETLLSV IEVLPKETLR HEILNPLVSK
181AQLSQTVQSR LVSCKILGKL TNKFDAHTIK REILPLVKSL CQDVEYEVRS CMCRQLENIA
241QGIGTELTKS VVLPELIELS RDEGSSVRLA AFETLVNLLD IFDTDDRSQT ILPLVKSFCE
301KSFKADESIL ISLSFHLGKL CHGLYGIFTP DQHLRFLEFY KKLCTLGLQQ ENGHNENQIP
361PQILEQEKKY ISVRKNCAYN FPAMIVFVDP KNFHMELYST FFCLCHDPEV PVRYTIAICF
421YEVSKLLNSG VYLIHKELIT LLQDESLEVL DALIDHLPEI LELMSTGGES SVQENKLSSL
481PDLIPALTAA EQRAAASLKW RTHEKLLQKY ACLPHVISSD QIYYRFLQRM FTIMMTNNVL
541PVQKAASRTL CIFLRYNRKQ EQRHEVIQKL IEQLGQGKSY WNRLRFLDTC EFIIEIFSKS
601FFCKYFFLPA IELTHDPVAN VRMKLCYLLP KVKSTLKIPA DKHLLQQLEM CVRKLLCQEK
661DKDVLAIVKR TVLELDRMEM SMDAFQKKFY EKDLLDQEKE REELLLLEME QLEKEKQQND
721GRPMSDKMFE KKRRDTKTPT QSLPKNIPIS VPGPSSVTPS TSKEIKKSKL IRSQSFNNQA
781FHAKYGNLEK CASKSSTTGY TTSVSGLGKT SVLSLADDSF RTRNASSVPS SFSPNTPLPS
841TSRGTGNSVD PKSSGSKDTQ PRKATLKSRK SNP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

873 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.