PPP4R4 Antibody
Catalog Number: LS-C834548
3 sizes / from $338.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PPP4R4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G91457-20
Catalog Number: LS-G91457
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PP4R4(PPP4R4) (NM_058237) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PPP4R4 | PP4R4 | KIAA1622
- Origin Species
- Human
- NCBI GenBank
- NM_020958
- SwissProt
- Q6NUP7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHPPPPAAAM DFSQNSLFGY MEDLQELTII ERPVRRSLKT PEEIERLTVD EDLSDIERAV61YLLSAGQDVQ GTSVIANLPF LMRQNPTETL RRVLPKVREA LHVAGVEMQL TAAMSFLTIL121QDESVSIHAY THSFLQVILL HLEHRDTGVS NAWLETLLSV IEVLPKETLR HEILNPLVSK181AQLSQTVQSR LVSCKILGKL TNKFDAHTIK REILPLVKSL CQDVEYEVRS CMCRQLENIA241QGIGTELTKS VVLPELIELS RDEGSSVRLA AFETLVNLLD IFDTDDRSQT ILPLVKSFCE301KSFKADESIL ISLSFHLGKL CHGLYGIFTP DQHLRFLEFY KKLCTLGLQQ ENGHNENQIP361PQILEQEKKY ISVRKNCAYN FPAMIVFVDP KNFHMELYST FFCLCHDPEV PVRYTIAICF421YEVSKLLNSG VYLIHKELIT LLQDESLEVL DALIDHLPEI LELMSTGGES SVQENKLSSL481PDLIPALTAA EQRAAASLKW RTHEKLLQKY ACLPHVISSD QIYYRFLQRM FTIMMTNNVL541PVQKAASRTL CIFLRYNRKQ EQRHEVIQKL IEQLGQGKSY WNRLRFLDTC EFIIEIFSKS601FFCKYFFLPA IELTHDPVAN VRMKLCYLLP KVKSTLKIPA DKHLLQQLEM CVRKLLCQEK661DKDVLAIVKR TVLELDRMEM SMDAFQKKFY EKDLLDQEKE REELLLLEME QLEKEKQQND721GRPMSDKMFE KKRRDTKTPT QSLPKNIPIS VPGPSSVTPS TSKEIKKSKL IRSQSFNNQA781FHAKYGNLEK CASKSSTTGY TTSVSGLGKT SVLSLADDSF RTRNASSVPS SFSPNTPLPS841TSRGTGNSVD PKSSGSKDTQ PRKATLKSRK SNPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
873 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
