Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP2R3A / PR130 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104342-100
Catalog Number: LS-G104342
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R3A (NM_181897), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PR130 | PR72 | PPP2R3 | PP2A | subunit B | R3 isoform | PPP2R3A
- Origin Species
- Human
- NCBI GenBank
- NM_002718
- SwissProt
- Q06190
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MMIKETSLRR DPDLRGELAF LARGCDFVLP SRFKKRLKSF QQTQIQNKPE KKPGTPLPPP61ATSPSSPRPL SPVPHVNNVV NAPLSINIPR FYFPEGLPDT CSNHEQTLSR IETAFMDIEE121QKADIYEMGK IAKVCGCPLY WKAPMFRAAG GEKTGFVTAQ SFIAMWRKLL NNHHDDASKF181ICLLAKPNCS SLEQEDFIPL LQDVVDTHPG LTFLKDAPEF HSRYITTVIQ RIFYTVNRSW241SGKITSTEIR KSNFLQTLAL LEEEEDINQI TDYFSYEHFY VIYCKFWELD TDHDLYISQA301DLSRYNDQAS SSRIIERIFS GAVTRGKTIQ KEGRMSYADF VWFLISEEDK RNPTSIEYWF361RCMDVDGDGV LSMYELEYFY EEQCERMEAM GIEPLPFHDL LCQMLDLVKP AVDGKITLRD421LKRCRMAHIF YDTFFNLEKY LDHEQRDPFA VQKDVENDGP EPSDWDRFAA EEYETLVAEE481SAQAQFQEGF EDYETDEPAS PSEFGNKSNK ILSASLPEKC GKLQSVDEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
529 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.