LSBio
Human PPP2R3A / PR130 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PPP2R3A / PR130 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G78356-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R3A (NM_181897) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PR130 | PR72 | PPP2R3 | PP2A | subunit B | R3 isoform | PPP2R3A
Origin Species
Human
NCBI GenBank
NM_002718
SwissProt
Q06190

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MMIKETSLRR DPDLRGELAF LARGCDFVLP SRFKKRLKSF QQTQIQNKPE KKPGTPLPPP
61ATSPSSPRPL SPVPHVNNVV NAPLSINIPR FYFPEGLPDT CSNHEQTLSR IETAFMDIEE
121QKADIYEMGK IAKVCGCPLY WKAPMFRAAG GEKTGFVTAQ SFIAMWRKLL NNHHDDASKF
181ICLLAKPNCS SLEQEDFIPL LQDVVDTHPG LTFLKDAPEF HSRYITTVIQ RIFYTVNRSW
241SGKITSTEIR KSNFLQTLAL LEEEEDINQI TDYFSYEHFY VIYCKFWELD TDHDLYISQA
301DLSRYNDQAS SSRIIERIFS GAVTRGKTIQ KEGRMSYADF VWFLISEEDK RNPTSIEYWF
361RCMDVDGDGV LSMYELEYFY EEQCERMEAM GIEPLPFHDL LCQMLDLVKP AVDGKITLRD
421LKRCRMAHIF YDTFFNLEKY LDHEQRDPFA VQKDVENDGP EPSDWDRFAA EEYETLVAEE
481SAQAQFQEGF EDYETDEPAS PSEFGNKSNK ILSASLPEKC GKLQSVDEE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

529 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.