LSBio
PPP1R13B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP1R13B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPP1R13B antibodies →
$725.00each
Catalog Number: LS-G113317-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ASPP1(PPP1R13B) (NM_015316), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASPP1 | p53BP2-like | p85 | KIAA0771 | PPP1R13B
Origin Species
Human
NCBI GenBank
NM_015316
SwissProt
Q96KQ4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
119.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMPMILTVFL SNNEQILTEV PITPETTCRD VVEFCKEPGE GSCHLAEVWR GNERPIPFDH
61MMYEHLQKWG PRREEVKFFL RHEDSPTENS EQGGRQTQEQ RTQRNVINVP GEKRTENGVG
121NPRVELTLSE LQDMAARQQQ QIENQQQMLV AKEQRLHFLK QQERRQQQSI SENEKLQKLK
181ERVEAQENKL KKIRAMRGQV DYSKIMNGNL SAEIERFSAM FQEKKQEVQT AILRVDQLSQ
241QLEDLKKGKL NGFQSYNGKL TGPAAVELKR LYQELQIRNQ LNQEQNSKLQ QQKELLNKRN
301MEVAMMDKRI SELRERLYGK KIQLNRVNGT SSPQSPLSTS GRVAAVGPYI QVPSAGSFPV
361LGDPIKPQSL SIASNAAHGR SKSANDGNWP TLKQNSSSSV KPVQVAGADW KDPSVEGSVK
421QGTVSSQPVP FSALGPTEKP GIEIGKVPPP IPGVGKQLPP SYGTYPSPTP LGPGSTSSLE
481RRKEGSLPRP SAGLPSRQRP TLLPATGSTP QPGSSQQIQQ RISVPPSPTY PPAGPPAFPA
541GDSKPELPLT VAIRPFLADK GSRPQSPRKG PQTVNSSSIY SMYLQQATPP KNYQPAAHSA
601LNKSVKAVYG KPVLPSGSTS PSPLPFLHGS LSTGTPQPQP PSESTEKEPE QDGPAAPADG
661STVESLPRPL SPTKLTPIVH SPLRYQSDAD LEALRRKLAN APRPLKKRSS ITEPEGPGGP
721NIQKLLYQRF NTLAGGMEGT PFYQPSPSQD FMGTLADVDN GNTNANGNLE ELPPAQPTAP
781LPAEPAPSSD ANDNELPSPE PEELICPQTT HQTAEPAEDN NNNVATVPTT EQIPSPVAEA
841PSPGEEQVPP APLPPASHPP ATSTNKRTNL KKPNSERTGH GLRVRFNPLA LLLDASLEGE
901FDLVQRIIYE VEDPSKPNDE GITPLHNAVC AGHHHIVKFL LDFGVNVNAA DSDGWTPLHC
961AASCNSVHLC KQLVESGAAI FASTISDIET AADKCEEMEE GYIQCSQFLY GVQEKLGVMN
1021KGVAYALWDY EAQNSDELSF HEGDALTILR RKDESETEWW WARLGDREGY VPKNLLGLYP
1081RIKPRQRTLA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1090 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.