Anti-ASPP1 Antibody - Western Blot. Western blot of affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1000 dilution. The identity of the lower MW band at approximately 50kD is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.