Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP1CA / PP1-Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121860-100
Catalog Number: LS-G121860
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP1A(PPP1CA) (NM_001008709), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PP-1A | pp1C alpha | PP1 alpha | PPP1CA | PP1-Alpha | PP1alpha | PPP1A | Protein phosphatase 1 alpha
- Origin Species
- Human
- NCBI GenBank
- NM_002708
- SwissProt
- P62136
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDSEKLNLD SIIGRLLEGS RVLTPHCAPV QGSRPGKNVQ LTENEIRGLC LKSREIFLSQ61PILLELEAPL KICGDIHGQY YDLLRLFEYG GFPPESNYLF LGDYVDRGKQ SLETICLLLA121YKIKYPENFF LLRGNHECAS INRIYGFYDE CKRRYNIKLW KTFTDCFNCL PIAAIVDEKI181FCCHGGLSPD LQSMEQIRRI MRPTDVPDQG LLCDLLWSDP DKDVQGWGEN DRGVSFTFGA241EVVAKFLHKH DLDLICRAHQ VVEDGYEFFA KRQLVTLFSA PNYCGEFDNA GAMMSVDETL301MCSFQILKPA DKNKGKYGQF SGLNPGGRPI TPPRNSAKAK KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
341 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.