PPP1CA / PP1-Alpha Antibody
Catalog Number: LS-C131250-100
100 µg / $576.00
Antibody: Mouse Monoclonal
Applications: Immunocytochemistry, Western Blot
Reactivity: Human, Mouse, Rabbit, Rat
Format: Lyophilized
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PPP1CA / PP1-Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G78748-20
Catalog Number: LS-G78748
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPP1A(PPP1CA) (NM_001008709) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PP-1A | pp1C alpha | PP1 alpha | PPP1CA | PP1-Alpha | PP1alpha | PPP1A | Protein phosphatase 1 alpha
- Origin Species
- Human
- NCBI GenBank
- NM_002708
- SwissProt
- P62136
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDSEKLNLD SIIGRLLEGS RVLTPHCAPV QGSRPGKNVQ LTENEIRGLC LKSREIFLSQ61PILLELEAPL KICGDIHGQY YDLLRLFEYG GFPPESNYLF LGDYVDRGKQ SLETICLLLA121YKIKYPENFF LLRGNHECAS INRIYGFYDE CKRRYNIKLW KTFTDCFNCL PIAAIVDEKI181FCCHGGLSPD LQSMEQIRRI MRPTDVPDQG LLCDLLWSDP DKDVQGWGEN DRGVSFTFGA241EVVAKFLHKH DLDLICRAHQ VVEDGYEFFA KRQLVTLFSA PNYCGEFDNA GAMMSVDETL301MCSFQILKPA DKNKGKYGQF SGLNPGGRPI TPPRNSAKAK KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
341 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
