LSBio
PPM1J Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPM1J Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPM1J antibodies →
$504.00each
Catalog Number: LS-G116133-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPM1J (NM_005167), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PP2C-zeta | PP2CZ | PPP2CZ | Protein phosphatase 2C zeta | RP11-426L16.5 | Transforming protein rhoc | PPM1J | Protein phosphatase 1J | PP2Czeta
Origin Species
Human
NCBI GenBank
AX400071
SwissProt
Q5JR12

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLNRVRSAVA HLVSSGGAPP PRPKSPDLPN AASAPPAAAP EAPRSPPAKA GSGSATPAKA
61VEARASFSRP TFLQLSPGGL RRADDHAGRA VQSPPDTGRR LPWSTGYAEV INAGKSRHNE
121DQACCEVVYV EGRRSVTGVP REPSRGQGLC FYYWGLFDGH AGGGAAEMAS RLLHRHIREQ
181LKDLVEILQD PSPPPLCLPT TPGTPDSSDP SHLLGPQSCW SSQKEVSHES LVVGAIENAF
241QLMDEQMARE RRGHQVEGGC CALVVIYLLG KVYVANAGDS RAIIVRNGEI IPMSREFTPE
301TERQRLQLLG FLKPELLGSE FTHLEFPRRV LPKELGQRML YRDQNMTGWA YKKIELEDLR
361FPLVCGEGKK ARVMATIGVT RGLGDHSLKV CSSTLPIKPF LSCFPEVRVY DLTQYEHCPD
421DVLVLGTDGL WDVTTDCEVA ATVDRVLSAY EPNDHSRYTA LAQALVLGAR GTPRDRGWRL
481PNNKLGSGDD ISVFVIPLGG PGSYS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

505 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.