PPM1J Antibody
Catalog Number: LS-C155212-100
100 µL (1 mg/mL) / $459.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PPM1J Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G79295-20
Catalog Number: LS-G79295
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPM1J (NM_005167) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PP2C-zeta | PP2CZ | PPP2CZ | Protein phosphatase 2C zeta | RP11-426L16.5 | Transforming protein rhoc | PPM1J | Protein phosphatase 1J | PP2Czeta
- Origin Species
- Human
- NCBI GenBank
- AX400071
- SwissProt
- Q5JR12
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLNRVRSAVA HLVSSGGAPP PRPKSPDLPN AASAPPAAAP EAPRSPPAKA GSGSATPAKA61VEARASFSRP TFLQLSPGGL RRADDHAGRA VQSPPDTGRR LPWSTGYAEV INAGKSRHNE121DQACCEVVYV EGRRSVTGVP REPSRGQGLC FYYWGLFDGH AGGGAAEMAS RLLHRHIREQ181LKDLVEILQD PSPPPLCLPT TPGTPDSSDP SHLLGPQSCW SSQKEVSHES LVVGAIENAF241QLMDEQMARE RRGHQVEGGC CALVVIYLLG KVYVANAGDS RAIIVRNGEI IPMSREFTPE301TERQRLQLLG FLKPELLGSE FTHLEFPRRV LPKELGQRML YRDQNMTGWA YKKIELEDLR361FPLVCGEGKK ARVMATIGVT RGLGDHSLKV CSSTLPIKPF LSCFPEVRVY DLTQYEHCPD421DVLVLGTDGL WDVTTDCEVA ATVDRVLSAY EPNDHSRYTA LAQALVLGAR GTPRDRGWRL481PNNKLGSGDD ISVFVIPLGG PGSYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
505 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
