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PPFIBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPFIBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPFIBP1 antibodies →
$725.00each
Catalog Number: LS-G117196-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPFIBP1 (NM_003622), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSGT2 | Liprin-beta-1 | PPFIBP1 | L2 | Liprin related protein | Liprin-beta 1 | HSgt2p | KIAA1230 | SGT2
Origin Species
Human
NCBI GenBank
NM_003622
SwissProt
Q86W92

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMSDASDMLA AALEQMDGII AGSKALEYSN GIFDCQSPTS PFMGSLRALH LVEDLRGLLE
61MMETDEKEGL RCQIPDSTAE TLVEWLQSQM TNGHLPGNGD VYQERLARLE NDKESLVLQV
121SVLTDQVEAQ GEKIRDLEFC LEEHREKLNA TEEMLQQELL SRTSLETQKL DLMAEISNLK
181LKLTAVEKDR LDYEDKFRDT EGLIQEINDL RLKVSEMDSE RLQYEKKLKS TKDELASLKE
241QLEEKESEVK RLQEKLVCKM KGEGVEIVDR DENFKKKLKE KNIEVQKMKK AVESLMAANE
301EKDRKIEDLR QCLNRYKKMQ DTVVLAQGKK GKDGEYEELL NSSSISSLLD AQGFSDLEKS
361PSPTPVMGSP SCDPFNTSVP EEFHTTILQV SIPSLLPATV SMETSEKSKL TPKPETSFEE
421NDGNIILGAT VDTQLCDKLL TSSLQKSSSL GNLKKETSDG EKETIQKTSE DRAPAESRPF
481GTLPPRPPGQ DTSMDDNPFG TRKVRSSFGR GFFKIKSNKR TASAPNLDRK RSASAPTLAE
541TEKETAEHLD LAGASSRPKD SQRNSPFQIP PPSPDSKKKS RGIMKLFGKL RRSQSTTFNP
601DDMSEPEFKR GGTRATAGPR LGWSRDLGQS NSDLDMPFAK WTKEQVCNWL MEQGLGSYLN
661SGKHWIASGQ TLLQASQQDL EKELGIKHSL HRKKLQLALQ ALGSEEETNH GKLDFNWVTR
721WLDDIGLPQY KTQFDEGRVD GRMLHYMTVD DLLSLKVVSV LHHLSIKRAI QVLRINNFEP
781NCLRRRPSDE NTIAPSEVQK WTNHRVMEWL RSVDLAEYAP NLRGSGVHGG LMVLEPRFNV
841ETMAQLLNIP PNKTLLRRHL ATHFNLLIGA EAQHQKRDAM ELPDYVLLTA TAKVKPKKLA
901FSNFGNLRKK KQEDGEEYVC PMELGQASGS ASKKGFKPGL DMHLYEEDDL DRLEQMEDSE
961GTVRQIGAFS EGINNLTHML KEDDMFKDFA ARSPSASITD EDSNV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1005 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.