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Human PPFIBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PPFIBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PPFIBP1 antibodies →
$220.00each
Catalog Number: LS-G81298-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PPFIBP1 (NM_003622) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSGT2 | Liprin-beta-1 | PPFIBP1 | L2 | Liprin related protein | Liprin-beta 1 | HSgt2p | KIAA1230 | SGT2
Origin Species
Human
NCBI GenBank
NM_003622
SwissProt
Q86W92

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMSDASDMLA AALEQMDGII AGSKALEYSN GIFDCQSPTS PFMGSLRALH LVEDLRGLLE
61MMETDEKEGL RCQIPDSTAE TLVEWLQSQM TNGHLPGNGD VYQERLARLE NDKESLVLQV
121SVLTDQVEAQ GEKIRDLEFC LEEHREKLNA TEEMLQQELL SRTSLETQKL DLMAEISNLK
181LKLTAVEKDR LDYEDKFRDT EGLIQEINDL RLKVSEMDSE RLQYEKKLKS TKDELASLKE
241QLEEKESEVK RLQEKLVCKM KGEGVEIVDR DENFKKKLKE KNIEVQKMKK AVESLMAANE
301EKDRKIEDLR QCLNRYKKMQ DTVVLAQGKK GKDGEYEELL NSSSISSLLD AQGFSDLEKS
361PSPTPVMGSP SCDPFNTSVP EEFHTTILQV SIPSLLPATV SMETSEKSKL TPKPETSFEE
421NDGNIILGAT VDTQLCDKLL TSSLQKSSSL GNLKKETSDG EKETIQKTSE DRAPAESRPF
481GTLPPRPPGQ DTSMDDNPFG TRKVRSSFGR GFFKIKSNKR TASAPNLDRK RSASAPTLAE
541TEKETAEHLD LAGASSRPKD SQRNSPFQIP PPSPDSKKKS RGIMKLFGKL RRSQSTTFNP
601DDMSEPEFKR GGTRATAGPR LGWSRDLGQS NSDLDMPFAK WTKEQVCNWL MEQGLGSYLN
661SGKHWIASGQ TLLQASQQDL EKELGIKHSL HRKKLQLALQ ALGSEEETNH GKLDFNWVTR
721WLDDIGLPQY KTQFDEGRVD GRMLHYMTVD DLLSLKVVSV LHHLSIKRAI QVLRINNFEP
781NCLRRRPSDE NTIAPSEVQK WTNHRVMEWL RSVDLAEYAP NLRGSGVHGG LMVLEPRFNV
841ETMAQLLNIP PNKTLLRRHL ATHFNLLIGA EAQHQKRDAM ELPDYVLLTA TAKVKPKKLA
901FSNFGNLRKK KQEDGEEYVC PMELGQASGS ASKKGFKPGL DMHLYEEDDL DRLEQMEDSE
961GTVRQIGAFS EGINNLTHML KEDDMFKDFA ARSPSASITD EDSNV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1005 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.