Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPARG / PPAR Gamma Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107269-100
Catalog Number: LS-G107269
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPAR gamma (PPARG) (NM_138712), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NR1C3 | PPAR gamma | PPARG | PPAR gamma 2 | PPARG1 | PPARG2 | PPARgamma | CIMT1 | GLM1 | PPAR-gamma
- Origin Species
- Human
- NCBI GenBank
- NM_015869
- SwissProt
- P37231
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTMVDTEMPF WPTNFGISSV DLSVMEDHSH SFDIKPFTTV DFSSISTPHY EDIPFTRTDP61VVADYKYDLK LQEYQSAIKV EPASPPYYSE KTQLYNKPHE EPSNSLMAIE CRVCGDKASG121FHYGVHACEG CKGFFRRTIR LKLIYDRCDL NCRIHKKSRN KCQYCRFQKC LAVGMSHNAI181RFGRMPQAEK EKLLAEISSD IDQLNPESAD LRALAKHLYD SYIKSFPLTK AKARAILTGK241TTDKSPFVIY DMNSLMMGED KIKFKHITPL QEQSKEVAIR IFQGCQFRSV EAVQEITEYA301KSIPGFVNLD LNDQVTLLKY GVHEIIYTML ASLMNKDGVL ISEGQGFMTR EFLKSLRKPF361GDFMEPKFEF AVKFNALELD DSDLAIFIAV IILSGDRPGL LNVKPIEDIQ DNLLQALELQ421LKLNHPESSQ LFAKLLQKMT DLRQIVTEHV QLLQVIKKTE TDMSLHPLLQ EIYKDLYTRT481RPLEQKLISE EDLAANDILD YKDDDDKV
508 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.