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POM121C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

POM121C Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all POM121C antibodies →
$725.00each
Catalog Number: LS-G119083-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POM121C (NM_001099415), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
nuclear envelope pore membrane protein POM 121C | POM121 membrane glycoprotein C | nuclear pore membrane protein 121-2 | pore membrane protein of 121 kDa C
Origin Species
Human
NCBI GenBank
NM_001099415

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVCSPVTVRI APPDRRFSRS AIPEQIISST LSSPSSNAPD PCAKETVLSA LKEKKKKRTV
61EEEDQIFLDG QENKRRRHDS SGSGHSAFEP LVASGVPASF VPKPGSLKRG LNSQSSDDHL
121NKRSRSSSMS SLTGAYTSGI PSSSRNAITS SYSSTRGISQ LWKRNGPSSS PFSSPASSRS
181QTPERPAKKI REEELCHHSS SSTPLAADKE SQGEKAADTT PRKKQNSNSQ STPGSSGQRK
241RKVQLLPSRR GEQLTLPPPP QLGYSITAED LDLEKKASLQ WFNQALEDKS DAASNSVTET
301PPTTQPSFTF TLPAAATASP PTSLLAPSTN PLLESLKKMQ TPPSLPPCPE SAGAATTEAL
361SPPKTPSLLP PLGLSQSGPP GLLPSPSFDS KPPTTLLGLI PAPSMVPATD TKAPPTLQAE
421TATKPQATSA PSPAPKQSFL FGTQNTSPSS PAAPAASSAS PMFKPIFTAP PKSEKEGPTP
481PGPSVTATAP SSSSLPTTTS TTAPTFQPVF SSMGPPASVP LPAPFFKQTT TPATAPTTTA
541PLFTGLASAT SAVAPITSAS PSTDSASKPA FGFGINSVSS SSVSTTTSTA TAASQPFLFG
601APQASAASFT PAMGSIFQFG KPPALPTTTT VTTFSQSLPT AVPTATSSSA ADFSGFGSTL
661ATSAPATSSQ PTLTFSNTST PTFNIPFGSS AKSPLPSYPG ANPQPAFGAA EGQPPGAAKP
721ALTPSFGSSF TFGNSAAPAP ATAPTPAPAS TIKIVPAHVP TPIQPTFGGA THSAFGLKAT
781ASAFGAPASS QPAFGGSTAV FSFGAATSSG FGATTQTASS GSSSSVFGST TPSPFTFGGS
841AAPAGSGSFG INVATPGSSA TTGAFSFGAG QSGSTATSTP FTGGLGQNAL GTTGQSTPFA
901FNVGSTTESK PVFGGTATPT FGQNTPAPGV GTSGSSLSFG ASSAPAQGFV GVGPFGSAAP
961SFSIGAGSKT PGARQRLQAR RQHTRKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

987 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.