POM121C Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G119083-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human POM121C Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95720-20
Catalog Number: LS-G95720
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human POM121C (NM_001099415) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- nuclear envelope pore membrane protein POM 121C | POM121 membrane glycoprotein C | nuclear pore membrane protein 121-2 | pore membrane protein of 121 kDa C
- Origin Species
- Human
- NCBI GenBank
- NM_001099415
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVCSPVTVRI APPDRRFSRS AIPEQIISST LSSPSSNAPD PCAKETVLSA LKEKKKKRTV61EEEDQIFLDG QENKRRRHDS SGSGHSAFEP LVASGVPASF VPKPGSLKRG LNSQSSDDHL121NKRSRSSSMS SLTGAYTSGI PSSSRNAITS SYSSTRGISQ LWKRNGPSSS PFSSPASSRS181QTPERPAKKI REEELCHHSS SSTPLAADKE SQGEKAADTT PRKKQNSNSQ STPGSSGQRK241RKVQLLPSRR GEQLTLPPPP QLGYSITAED LDLEKKASLQ WFNQALEDKS DAASNSVTET301PPTTQPSFTF TLPAAATASP PTSLLAPSTN PLLESLKKMQ TPPSLPPCPE SAGAATTEAL361SPPKTPSLLP PLGLSQSGPP GLLPSPSFDS KPPTTLLGLI PAPSMVPATD TKAPPTLQAE421TATKPQATSA PSPAPKQSFL FGTQNTSPSS PAAPAASSAS PMFKPIFTAP PKSEKEGPTP481PGPSVTATAP SSSSLPTTTS TTAPTFQPVF SSMGPPASVP LPAPFFKQTT TPATAPTTTA541PLFTGLASAT SAVAPITSAS PSTDSASKPA FGFGINSVSS SSVSTTTSTA TAASQPFLFG601APQASAASFT PAMGSIFQFG KPPALPTTTT VTTFSQSLPT AVPTATSSSA ADFSGFGSTL661ATSAPATSSQ PTLTFSNTST PTFNIPFGSS AKSPLPSYPG ANPQPAFGAA EGQPPGAAKP721ALTPSFGSSF TFGNSAAPAP ATAPTPAPAS TIKIVPAHVP TPIQPTFGGA THSAFGLKAT781ASAFGAPASS QPAFGGSTAV FSFGAATSSG FGATTQTASS GSSSSVFGST TPSPFTFGGS841AAPAGSGSFG INVATPGSSA TTGAFSFGAG QSGSTATSTP FTGGLGQNAL GTTGQSTPFA901FNVGSTTESK PVFGGTATPT FGQNTPAPGV GTSGSSLSFG ASSAPAQGFV GVGPFGSAAP961SFSIGAGSKT PGARQRLQAR RQHTRKKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
987 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
