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PNPLA7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PNPLA7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PNPLA7 antibodies →
$725.00each
Catalog Number: LS-G119261-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PNPLA7 (NM_001098537), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001098537

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
148.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEEKDDSPQ ADFCLGTALH SWGLWFTEEG SPSTMLTGIA VGALLALALV GVLILFMFRR
61LRQFRQAQPT PQYRFRKRDK VMFYGRKIMR KVTTLPNTLV ENTALPRQRA RKRTKVLSLA
121KRILRFKKEY PALQPKEPPP SLLEADLTEF DVKNSHLPSE VLYMLKNVRV LGHFEKPLFL
181ELCKHIVFVQ LQEGEHVFQP REPDPSICVV QDGRLEVCIQ DTDGTEVVVK EVLAGDSVHS
241LLSILDIITG HAAPYKTVSV RAAIPSTILR LPAAAFHGVF EKYPETLVRV VQIIMVRLQR
301VTFLALHNYL GLTTELFNAE SQAIPLVSVA SVAAGKAKKQ VFYGEEERLK KPPRLQESCD
361SDHGGGRPAA AGPLLKRSHS VPAPSIRKQI LEELEKPGAG DPDPSAPQGG PGSATSDLGM
421ACDRARVFLH SDEHPGSSVA SKSRKSVMVA EIPSTVSQHS ESHTDETLAS RKSDAIFRAA
481KKDLLTLMKL EDSSLLDGRV ALLHVPAGTV VSRQGDQDAS ILFVVSGLLH VYQRKIGSQE
541DTCLFLTRPG EMVGQLAVLT GEPLIFTVKA NRDCSFLSIS KAHFYEIMRK QPTVVLGVAH
601TVVKRMSSFV RQIDFALDWV EVEAGRAIYR QGDKSDCTYI MLSGRLRSVI RKDDGKKRLA
661GEYGRGDLVG VVETLTHQAR ATTVHAVRDS ELAKLPAGAL TSIKRRYPQV VTRLIHLLGE
721KILGSLQQGP VTGHQLGLPT EGSKWDLGNP AVNLSTVAVM PVSEEVPLTA FALELEHALS
781AIGPTLLLTS DNIKRRLGSA ALDSVHEYRL SSWLGQQEDT HRIVLYQVDG TLTPWTQRCV
841RQADCILIVG LGDQEPTVGE LERMLESTAV RAQKQLILLH REEGPAPART VEWLNMRSWC
901SGHLHLCCPR RVFSRRSLPK LVEMYKHVFQ RPPDRHSDFS RLARVLTGNA IALVLGGGGA
961RGCAQVGVLK ALAECGIPVD MVGGTSIGAF VGALYSEERN YSQMRIRAKQ WAEGMTSLMK
1021AALDLTYPIT SMFSGAGFNS SIFSVFKDQQ IEDLWIPYFA ITTDITASAM RVHTDGSLWW
1081YVRASMSLSG YMPPLCDPKD GHLLMDGGYI NNLPADVARS MGAKVVIAID VGSRDETDLT
1141NYGDALSGWW LLWKRWNPLA TKVKVLNMAE IQTRLAYVCC VRQLEVVKSS DYCEYLRPPI
1201DSYSTLDFGK FNEICEVGYQ HGRTVFDIWG RSGVLEKMLR DQQGPSKKPA SAVLTCPNAS
1261FTDLAEIVSR IEPAKPAMVD DESDYQTEYE EELLDVPRDA YADFQSTSAQ QGSDLEDESS
1321LRHRHPSLAF PKLSEGSSDQ DG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1342 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.