PNPLA7 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G119261-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PNPLA7 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G96133-20
Catalog Number: LS-G96133
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PNPLA7 (NM_001098537) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001098537
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 148.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEEKDDSPQ ADFCLGTALH SWGLWFTEEG SPSTMLTGIA VGALLALALV GVLILFMFRR61LRQFRQAQPT PQYRFRKRDK VMFYGRKIMR KVTTLPNTLV ENTALPRQRA RKRTKVLSLA121KRILRFKKEY PALQPKEPPP SLLEADLTEF DVKNSHLPSE VLYMLKNVRV LGHFEKPLFL181ELCKHIVFVQ LQEGEHVFQP REPDPSICVV QDGRLEVCIQ DTDGTEVVVK EVLAGDSVHS241LLSILDIITG HAAPYKTVSV RAAIPSTILR LPAAAFHGVF EKYPETLVRV VQIIMVRLQR301VTFLALHNYL GLTTELFNAE SQAIPLVSVA SVAAGKAKKQ VFYGEEERLK KPPRLQESCD361SDHGGGRPAA AGPLLKRSHS VPAPSIRKQI LEELEKPGAG DPDPSAPQGG PGSATSDLGM421ACDRARVFLH SDEHPGSSVA SKSRKSVMVA EIPSTVSQHS ESHTDETLAS RKSDAIFRAA481KKDLLTLMKL EDSSLLDGRV ALLHVPAGTV VSRQGDQDAS ILFVVSGLLH VYQRKIGSQE541DTCLFLTRPG EMVGQLAVLT GEPLIFTVKA NRDCSFLSIS KAHFYEIMRK QPTVVLGVAH601TVVKRMSSFV RQIDFALDWV EVEAGRAIYR QGDKSDCTYI MLSGRLRSVI RKDDGKKRLA661GEYGRGDLVG VVETLTHQAR ATTVHAVRDS ELAKLPAGAL TSIKRRYPQV VTRLIHLLGE721KILGSLQQGP VTGHQLGLPT EGSKWDLGNP AVNLSTVAVM PVSEEVPLTA FALELEHALS781AIGPTLLLTS DNIKRRLGSA ALDSVHEYRL SSWLGQQEDT HRIVLYQVDG TLTPWTQRCV841RQADCILIVG LGDQEPTVGE LERMLESTAV RAQKQLILLH REEGPAPART VEWLNMRSWC901SGHLHLCCPR RVFSRRSLPK LVEMYKHVFQ RPPDRHSDFS RLARVLTGNA IALVLGGGGA961RGCAQVGVLK ALAECGIPVD MVGGTSIGAF VGALYSEERN YSQMRIRAKQ WAEGMTSLMK1021AALDLTYPIT SMFSGAGFNS SIFSVFKDQQ IEDLWIPYFA ITTDITASAM RVHTDGSLWW1081YVRASMSLSG YMPPLCDPKD GHLLMDGGYI NNLPADVARS MGAKVVIAID VGSRDETDLT1141NYGDALSGWW LLWKRWNPLA TKVKVLNMAE IQTRLAYVCC VRQLEVVKSS DYCEYLRPPI1201DSYSTLDFGK FNEICEVGYQ HGRTVFDIWG RSGVLEKMLR DQQGPSKKPA SAVLTCPNAS1261FTDLAEIVSR IEPAKPAMVD DESDYQTEYE EELLDVPRDA YADFQSTSAQ QGSDLEDESS1321LRHRHPSLAF PKLSEGSSDQ DGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1342 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
