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PML Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PML Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108354-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PML Protein(PML) (NM_033250), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PP8675 | Promyelocytic leukemia | Protein PML | TRIM19 | PML | Promyelocytic leukemia protein | RING finger protein 71 | MYL | RNF71
Origin Species
Human
NCBI GenBank
NM_002675
SwissProt
P29590

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPAPARSPR PQQDPARPQE PTMPPPETPS EGRQPSPSPS PTERAPASEE EFQFLRCQQC
61QAEAKCPKLL PCLHTLCSGC LEASGMQCPI CQAPWPLGAD TPALDNVFFE SLQRRLSVYR
121QIVDAQAVCT RCKESADFWC FECEQLLCAK CFEAHQWFLK HEARPLAELR NQSVREFLDG
181TRKTNNIFCS NPNHRTPTLT SIYCRGCSKP LCCSCALLDS SHSDLKCDIS AEIQQRQEEL
241DAMTQALQEQ DSAFGAVHAQ MHAVVGQLGR ARAETEELIR ERVRQVVAHV RAQERELLEA
301VDARYQRDYE EMASRLGRLD AVLQRIRTGS ALVQRMKCYA SDQEVLDMHG FLRQALCRLR
361QEEPQSLQAA VRTDGFDEFK VRLQDLSSCI TQGKDAAVSK KASPEAASTP RDPIDVDLDV
421SNTTTAQKRK CSQTQCPRKV IKMESEEGKE ARLARSSPEQ PRPSTSKAVS PPHLDGPPSP
481RSPVIGSEVF LPNSNHVASG AGEAEERVVV ISSSEDSDAE NSCMEPMETA EPQSSPAHSS
541PAHSSPAHSS PVQSLLRAQG ASSLPCGTYH PPAWPPHQPA EQAATPDAEP HSEPPDHQER
601PAVHRGIRYL LYRAQRAIRL RHALRLHPQL HRAPIRTWSP HVVQASTPAI TGPLNHPANA
661QEHPAQLQRG ISPPHRIRGA VRSRSRSLRG SSHLSQWLNN FFALPFSSMA SQLDMSSVVG
721AGEGRAQTLG AVVPPGDSVR GSMEASQVQV PLEASPITFP PPCAPERPPI SPVPGARQAG
781L
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

781 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.