PML Antibody
Catalog Number: LS-C126143-500
500 µg / $671.00
Antibody: Mouse Monoclonal
Applications: Western Blot
Reactivity: Human
Format: Lyophilized
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PML Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G88781-20
Catalog Number: LS-G88781
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PML Protein(PML) (NM_033250) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PP8675 | Promyelocytic leukemia | Protein PML | TRIM19 | PML | Promyelocytic leukemia protein | RING finger protein 71 | MYL | RNF71
- Origin Species
- Human
- NCBI GenBank
- NM_002675
- SwissProt
- P29590
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPAPARSPR PQQDPARPQE PTMPPPETPS EGRQPSPSPS PTERAPASEE EFQFLRCQQC61QAEAKCPKLL PCLHTLCSGC LEASGMQCPI CQAPWPLGAD TPALDNVFFE SLQRRLSVYR121QIVDAQAVCT RCKESADFWC FECEQLLCAK CFEAHQWFLK HEARPLAELR NQSVREFLDG181TRKTNNIFCS NPNHRTPTLT SIYCRGCSKP LCCSCALLDS SHSDLKCDIS AEIQQRQEEL241DAMTQALQEQ DSAFGAVHAQ MHAVVGQLGR ARAETEELIR ERVRQVVAHV RAQERELLEA301VDARYQRDYE EMASRLGRLD AVLQRIRTGS ALVQRMKCYA SDQEVLDMHG FLRQALCRLR361QEEPQSLQAA VRTDGFDEFK VRLQDLSSCI TQGKDAAVSK KASPEAASTP RDPIDVDLDV421SNTTTAQKRK CSQTQCPRKV IKMESEEGKE ARLARSSPEQ PRPSTSKAVS PPHLDGPPSP481RSPVIGSEVF LPNSNHVASG AGEAEERVVV ISSSEDSDAE NSCMEPMETA EPQSSPAHSS541PAHSSPAHSS PVQSLLRAQG ASSLPCGTYH PPAWPPHQPA EQAATPDAEP HSEPPDHQER601PAVHRGIRYL LYRAQRAIRL RHALRLHPQL HRAPIRTWSP HVVQASTPAI TGPLNHPANA661QEHPAQLQRG ISPPHRIRGA VRSRSRSLRG SSHLSQWLNN FFALPFSSMA SQLDMSSVVG721AGEGRAQTLG AVVPPGDSVR GSMEASQVQV PLEASPITFP PPCAPERPPI SPVPGARQAG781LSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
781 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
