Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLXNB1 / Plexin-B1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117831-100
Catalog Number: LS-G117831
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Plexin B1(PLXNB1) (NM_002673), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0407 | Plexin 5 | Plexin B1 | PLXNB1 | Semaphorin receptor SEP | PLXN5 | SEP | Plexin-B1
- Origin Species
- Human
- NCBI GenBank
- NM_002673
- SwissProt
- O43157
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 232.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPALGPALLQ ALWAGWVLTL QPLPPTAFTP NGTYLQHLAR DPTSGTLYLG ATNFLFQLSP61GLQLEATVST GPVLDSRDCL PPVMPDECPQ AQPTNNPNQL LLVSPGALVV CGSVHQGVCE121QRRLGQLEQL LLRPERPGDT QYVAANDPAV STVGLVAQGL AGEPLLFVGR GYTSRGVGGG181IPPITTRALW PPDPQAAFSY EETAKLAVGR LSEYSHHFVS AFARGASAYF LFLRRDLQAQ241SRAFRAYVSR VCLRDQHYYS YVELPLACEG GRYGLIQAAA VATSREVAHG EVLFAAFSSA301APPTVGRPPS AAAGASGASA LCAFPLDEVD RLANRTRDAC YTREGRAEDG TEVAYIEYDV361NSDCAQLPVD TLDAYPCGSD HTPSPMASRV PLEATPILEW PGIQLTAVAV TMEDGHTIAF421LGDSQGQLHR VYLGPGSDGH PYSTQSIQQG SAVSRDLTFD GTFEHLYVMT QSTLLKVPVA481SCAQHLDCAS CLAHRDPYCG WCVLLGRCSR RSECSRGQGP EQWLWSFQPE LGCLQVAAMS541PANISREETR EVFLSVPDLP PLWPGESYSC HFGEHQSPAL LTGSGVMCPS PDPSEAPVLP601RGADYVSVSV ELRFGAVVIA KTSLSFYDCV AVTELRPSAQ CQACVSSRWG CNWCVWQHLC661THKASCDAGP MVASHQSPLV SPDPPARGGP SPSPPTAPKA LATPAPDTLP VEPGAPSTAT721ASDISPGASP SLLSPWGPWA GSGSISSPGS TGSPLHEEPS PPSPQNGPGT AVPAPTDFRP781SATPEDLLAS PLSPSEVAAV PPADPGPEAL HPTVPLDLPP ATVPATTFPG AMGSVKPALD841WLTREGGELP EADEWTGGDA PAFSTSTLLS GDGDSAELEG PPAPLILPSS LDYQYDTPGL901WELEEATLGA SSCPCVESVQ GSTLMPVHVE REIRLLGRNL HLFQDGPGDN ECVMELEGLE961VVVEARVECE PPPDTQCHVT CQQHQLSYEA LQPELRVGLF LRRAGRLRVD SAEGLHVVLY1021DCSVGHGDCS RCQTAMPQYG CVWCEGERPR CVTREACGEA EAVATQCPAP LIHSVEPLTG1081PVDGGTRVTI RGSNLGQHVQ DVLGMVTVAG VPCAVDAQEY EVSSSLVCIT GASGEEVAGA1141TAVEVPGRGR GVSEHDFAYQ DPKVHSIFPA RGPRAGGTRL TLNGSKLLTG RLEDIRVVVG1201DQPCHLLPEQ QSEQLRCETS PRPTPATLPV AVWFGATERR LQRGQFKYTL DPNITSAGPT1261KSFLSGGREI CVRGQNLDVV QTPRIRVTVV SRMLQPSQGL GRRRRVVPET ACSLGPSCSS1321QQFEEPCHVN SSQLITCRTP ALPGLPEDPW VRVEFILDNL VFDFATLNPT PFSYEADPTL1381QPLNPEDPTM PFRHKPGSVF SVEGENLDLA MSKEEVVAMI GDGPCVVKTL TRHHLYCEPP1441VEQPLPRHHA LREAPDSLPE FTVQMGNLRF SLGHVQYDGE SPGAFPVAAQ VGLGVGTSLL1501ALGVIIIVLM YRRKSKQALR DYKKVQIQLE NLESSVRDRC KKEFTDLMTE MTDLTSDLLG1561SGIPFLDYKV YAERIFFPGH RESPLHRDLG VPESRRPTVE QGLGQLSNLL NSKLFLTKFI1621HTLESQRTFS ARDRAYVASL LTVALHGKLE YFTDILRTLL SDLVAQYVAK NPKLMLRRTE1681TVVEKLLTNW MSICLYTFVR DSVGEPLYML FRGIKHQVDK GPVDSVTGKA KYTLNDNRLL1741REDVEYRPLT LNALLAVGPG AGEAQGVPVK VLDCDTISQA KEKMLDQLYK GVPLTQRPDP1801RTLDVEWRSG VAGHLILSDE DVTSEVQGLW RRLNTLQHYK VPDGATVALV PCLTKHVLRE1861NQDYVPGERT PMLEDVDEGG IRPWHLVKPS DEPEPPRPRR GSLRGGERER AKAIPEIYLT1921RLLSMKGTLQ KFVDDLFQVI LSTSRPVPLA VKYFFDLLDE QAQQHGISDQ DTIHIWKTNS1981LPLRFWINII KNPQFVFDVQ TSDNMDAVLL VIAQTFMDAC TLADHKLGRD SPINKLLYAR2041DIPRYKRMVE RYYADIRQTV PASDQEMNSV LAELSWNYSG DLGARVALHE LYKYINKYYD2101QIITALEEDG TAQKMQLGYR LQQIAAAVEN KVTDLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2135 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -80°C for long term storage.