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Human PLXNB1 / Plexin-B1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human PLXNB1 / Plexin-B1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PLXNB1 antibodies →
$220.00each
Catalog Number: LS-G79858-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Plexin B1(PLXNB1) (NM_002673) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0407 | Plexin 5 | Plexin B1 | PLXNB1 | Semaphorin receptor SEP | PLXN5 | SEP | Plexin-B1
Origin Species
Human
NCBI GenBank
NM_002673
SwissProt
O43157

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
232.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPALGPALLQ ALWAGWVLTL QPLPPTAFTP NGTYLQHLAR DPTSGTLYLG ATNFLFQLSP
61GLQLEATVST GPVLDSRDCL PPVMPDECPQ AQPTNNPNQL LLVSPGALVV CGSVHQGVCE
121QRRLGQLEQL LLRPERPGDT QYVAANDPAV STVGLVAQGL AGEPLLFVGR GYTSRGVGGG
181IPPITTRALW PPDPQAAFSY EETAKLAVGR LSEYSHHFVS AFARGASAYF LFLRRDLQAQ
241SRAFRAYVSR VCLRDQHYYS YVELPLACEG GRYGLIQAAA VATSREVAHG EVLFAAFSSA
301APPTVGRPPS AAAGASGASA LCAFPLDEVD RLANRTRDAC YTREGRAEDG TEVAYIEYDV
361NSDCAQLPVD TLDAYPCGSD HTPSPMASRV PLEATPILEW PGIQLTAVAV TMEDGHTIAF
421LGDSQGQLHR VYLGPGSDGH PYSTQSIQQG SAVSRDLTFD GTFEHLYVMT QSTLLKVPVA
481SCAQHLDCAS CLAHRDPYCG WCVLLGRCSR RSECSRGQGP EQWLWSFQPE LGCLQVAAMS
541PANISREETR EVFLSVPDLP PLWPGESYSC HFGEHQSPAL LTGSGVMCPS PDPSEAPVLP
601RGADYVSVSV ELRFGAVVIA KTSLSFYDCV AVTELRPSAQ CQACVSSRWG CNWCVWQHLC
661THKASCDAGP MVASHQSPLV SPDPPARGGP SPSPPTAPKA LATPAPDTLP VEPGAPSTAT
721ASDISPGASP SLLSPWGPWA GSGSISSPGS TGSPLHEEPS PPSPQNGPGT AVPAPTDFRP
781SATPEDLLAS PLSPSEVAAV PPADPGPEAL HPTVPLDLPP ATVPATTFPG AMGSVKPALD
841WLTREGGELP EADEWTGGDA PAFSTSTLLS GDGDSAELEG PPAPLILPSS LDYQYDTPGL
901WELEEATLGA SSCPCVESVQ GSTLMPVHVE REIRLLGRNL HLFQDGPGDN ECVMELEGLE
961VVVEARVECE PPPDTQCHVT CQQHQLSYEA LQPELRVGLF LRRAGRLRVD SAEGLHVVLY
1021DCSVGHGDCS RCQTAMPQYG CVWCEGERPR CVTREACGEA EAVATQCPAP LIHSVEPLTG
1081PVDGGTRVTI RGSNLGQHVQ DVLGMVTVAG VPCAVDAQEY EVSSSLVCIT GASGEEVAGA
1141TAVEVPGRGR GVSEHDFAYQ DPKVHSIFPA RGPRAGGTRL TLNGSKLLTG RLEDIRVVVG
1201DQPCHLLPEQ QSEQLRCETS PRPTPATLPV AVWFGATERR LQRGQFKYTL DPNITSAGPT
1261KSFLSGGREI CVRGQNLDVV QTPRIRVTVV SRMLQPSQGL GRRRRVVPET ACSLGPSCSS
1321QQFEEPCHVN SSQLITCRTP ALPGLPEDPW VRVEFILDNL VFDFATLNPT PFSYEADPTL
1381QPLNPEDPTM PFRHKPGSVF SVEGENLDLA MSKEEVVAMI GDGPCVVKTL TRHHLYCEPP
1441VEQPLPRHHA LREAPDSLPE FTVQMGNLRF SLGHVQYDGE SPGAFPVAAQ VGLGVGTSLL
1501ALGVIIIVLM YRRKSKQALR DYKKVQIQLE NLESSVRDRC KKEFTDLMTE MTDLTSDLLG
1561SGIPFLDYKV YAERIFFPGH RESPLHRDLG VPESRRPTVE QGLGQLSNLL NSKLFLTKFI
1621HTLESQRTFS ARDRAYVASL LTVALHGKLE YFTDILRTLL SDLVAQYVAK NPKLMLRRTE
1681TVVEKLLTNW MSICLYTFVR DSVGEPLYML FRGIKHQVDK GPVDSVTGKA KYTLNDNRLL
1741REDVEYRPLT LNALLAVGPG AGEAQGVPVK VLDCDTISQA KEKMLDQLYK GVPLTQRPDP
1801RTLDVEWRSG VAGHLILSDE DVTSEVQGLW RRLNTLQHYK VPDGATVALV PCLTKHVLRE
1861NQDYVPGERT PMLEDVDEGG IRPWHLVKPS DEPEPPRPRR GSLRGGERER AKAIPEIYLT
1921RLLSMKGTLQ KFVDDLFQVI LSTSRPVPLA VKYFFDLLDE QAQQHGISDQ DTIHIWKTNS
1981LPLRFWINII KNPQFVFDVQ TSDNMDAVLL VIAQTFMDAC TLADHKLGRD SPINKLLYAR
2041DIPRYKRMVE RYYADIRQTV PASDQEMNSV LAELSWNYSG DLGARVALHE LYKYINKYYD
2101QIITALEEDG TAQKMQLGYR LQQIAAAVEN KVTDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2135 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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