Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLA2G15 / ACS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114500-100
Catalog Number: LS-G114500
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LYPLA3(PLA2G15) (NM_012320), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 1-O-acylceramide synthase | ACS | Group XV phospholipase A2 | GXVPLA2 | LCAT-like lysophospholipase | LYPLA3 | Lysosomal phospholipase A2 | Phospholipase A2 | group XV | LLPL | LPLA2 | Lysophospholipase 3 | PLA2G15
- Origin Species
- Human
- NCBI GenBank
- NM_012320
- SwissProt
- Q8NCC3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLHLRPYRV GLLPDGLLFL LLLLMLLADP ALPAGRHPPV VLVPGDLGNQ LEAKLDKPTV61VHYLCSKKTE SYFTIWLNLE LLLPVIIDCW IDNIRLVYNK TSRATQFPDG VDVRVPGFGK121TFSLEFLDPS KSSVGSYFHT MVESLVGWGY TRGEDVRGAP YDWRRAPNEN GPYFLALREM181IEEMYQLYGG PVVLVAHSMG NMYTLYFLQR QPQAWKDKYI RAFVSLGAPW GGVAKTLRVL241ASGDNNRIPV IGPLKIREQQ RSAVSTSWLL PYNYTWSPEK VFVQTPTINY TLRDYRKFFQ301DIGFEDGWLM RQDTEGLVEA TMPPGVQLHC LYGTGVPTPD SFYYESFPDR DPKICFGDGD361GTVNLKSALQ CQAWQSRQEH QVLLQELPGS EHIEMLANAT TLAYLKRVLL GPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
412 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.