PLA2G15 / ACS Antibody
Catalog Number: LS-C830969
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PLA2G15 / ACS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G89720-20
Catalog Number: LS-G89720
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human LYPLA3(PLA2G15) (NM_012320) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 1-O-acylceramide synthase | ACS | Group XV phospholipase A2 | GXVPLA2 | LCAT-like lysophospholipase | LYPLA3 | Lysosomal phospholipase A2 | Phospholipase A2 | group XV | LLPL | LPLA2 | Lysophospholipase 3 | PLA2G15
- Origin Species
- Human
- NCBI GenBank
- NM_012320
- SwissProt
- Q8NCC3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLHLRPYRV GLLPDGLLFL LLLLMLLADP ALPAGRHPPV VLVPGDLGNQ LEAKLDKPTV61VHYLCSKKTE SYFTIWLNLE LLLPVIIDCW IDNIRLVYNK TSRATQFPDG VDVRVPGFGK121TFSLEFLDPS KSSVGSYFHT MVESLVGWGY TRGEDVRGAP YDWRRAPNEN GPYFLALREM181IEEMYQLYGG PVVLVAHSMG NMYTLYFLQR QPQAWKDKYI RAFVSLGAPW GGVAKTLRVL241ASGDNNRIPV IGPLKIREQQ RSAVSTSWLL PYNYTWSPEK VFVQTPTINY TLRDYRKFFQ301DIGFEDGWLM RQDTEGLVEA TMPPGVQLHC LYGTGVPTPD SFYYESFPDR DPKICFGDGD361GTVNLKSALQ CQAWQSRQEH QVLLQELPGS EHIEMLANAT TLAYLKRVLL GPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
412 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
