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PIWIL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PIWIL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G121944-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIWIL3 (NM_001008496), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HIWI3 | Piwi-like protein 3 | PIWIL3 | Piwi-like 3 (Drosophila)
Origin Species
Human
SwissProt
Q7Z3Z3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPGRARTRAR GRARRRESYQ QEAPGGPRAP GSATTQEPPQ LQSTPRPLQE EVPVVRPLQP
61RAARGGAGGG AQSQGVKEPG PEAGLHTAPL QERRIGGVFQ DLVVNTRQDM KHVKDSKTGS
121EGTVVQLLAN HFRVISRPQW VAYKYNVDYK PDIEDGNLRT ILLDQHRRKF GERHIFDGNS
181LLLSRPLKER RVEWLSTTKD KNIVKITVEF SKELTPTSPD CLRYYNILFR RTFKLLDFEQ
241VGRNYYTKKK AIQLYRHGTS LEIWLGYVTS VLQYENSITL CADVSHKLLR IETAYDFIKR
301TSAQAQTGNI REEVTNKLIG SIVLTKYNNK TYRVDDIDWK QNPEDTFNKS DGSKITYIDY
361YRQQHKEIVT VKKQPLLVSQ GRWKKGLTGT QREPILLIPQ LCHMTGLTDE ICKDYSIVKE
421LAKHTRLSPR RRHHTLKEFI NTLQDNKKVR ELLQLWDLKF DTNFLSVPGR VLKNANIVQG
481RRMVKANSQG DWSREIRELP LLNAMPLHSW LILYSRSSHR EAMSLKGHLQ SVTAPMGITM
541KPAEMIEVDG DANSYIDTLR KYTRPTLQMG MSCLLVFKVI CILPNDDKRR YDSIKRYLCT
601KCPIPSQCVV KKTLEKVQAR TIVTKIAQQM NCKMGGALWK VETDVQRTMF VGIDCFHDIV
661NRQKSIAGFV ASTNAELTKW YSQCVIQKTG EELVKELEIC LKAALDVWCK NESSMPHSVI
721VYRDGVGDGQ LQALLDHEAK KMSTYLKTIS PNNFTLAFIV VKKRINTRFF LKHGSNFQNP
781PPGTVIDVEL TRNEWYDFFI VSQSVQDGTV TPTHYNVIYD TIGLSPDTVQ RLTYCLCHMY
841YNLPGIIRVP APCHYAHKLA YLVGQSIHQE PNRSLSTRLF YL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

882 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.