Polyclonal Rabbit anti-Human PIWIL3 Antibody (WB)
Catalog Number: LS-C748265
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PIWIL3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G94173-20
Catalog Number: LS-G94173
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PIWIL3 (NM_001008496) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HIWI3 | Piwi-like protein 3 | PIWIL3 | Piwi-like 3 (Drosophila)
- Origin Species
- Human
- SwissProt
- Q7Z3Z3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGRARTRAR GRARRRESYQ QEAPGGPRAP GSATTQEPPQ LQSTPRPLQE EVPVVRPLQP61RAARGGAGGG AQSQGVKEPG PEAGLHTAPL QERRIGGVFQ DLVVNTRQDM KHVKDSKTGS121EGTVVQLLAN HFRVISRPQW VAYKYNVDYK PDIEDGNLRT ILLDQHRRKF GERHIFDGNS181LLLSRPLKER RVEWLSTTKD KNIVKITVEF SKELTPTSPD CLRYYNILFR RTFKLLDFEQ241VGRNYYTKKK AIQLYRHGTS LEIWLGYVTS VLQYENSITL CADVSHKLLR IETAYDFIKR301TSAQAQTGNI REEVTNKLIG SIVLTKYNNK TYRVDDIDWK QNPEDTFNKS DGSKITYIDY361YRQQHKEIVT VKKQPLLVSQ GRWKKGLTGT QREPILLIPQ LCHMTGLTDE ICKDYSIVKE421LAKHTRLSPR RRHHTLKEFI NTLQDNKKVR ELLQLWDLKF DTNFLSVPGR VLKNANIVQG481RRMVKANSQG DWSREIRELP LLNAMPLHSW LILYSRSSHR EAMSLKGHLQ SVTAPMGITM541KPAEMIEVDG DANSYIDTLR KYTRPTLQMG MSCLLVFKVI CILPNDDKRR YDSIKRYLCT601KCPIPSQCVV KKTLEKVQAR TIVTKIAQQM NCKMGGALWK VETDVQRTMF VGIDCFHDIV661NRQKSIAGFV ASTNAELTKW YSQCVIQKTG EELVKELEIC LKAALDVWCK NESSMPHSVI721VYRDGVGDGQ LQALLDHEAK KMSTYLKTIS PNNFTLAFIV VKKRINTRFF LKHGSNFQNP781PPGTVIDVEL TRNEWYDFFI VSQSVQDGTV TPTHYNVIYD TIGLSPDTVQ RLTYCLCHMY841YNLPGIIRVP APCHYAHKLA YLVGQSIHQE PNRSLSTRLF YLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
882 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
