LSBio
PIP5K1A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PIP5K1A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G125482-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIP5K1 alpha(PIP5K1A) (NM_001135636), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PIP5K1A | PIP5KIalpha | PtdIns(4)P-5-kinase 1 alpha | PIP5K1-alpha
Origin Species
Human
NCBI GenBank
NM_003557
SwissProt
Q99755

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MASASSGPSS SVGFSSFDPA VPSCTLSSAA SGIKRPMASE VPYASGMPIK KIGHRSVDSS
61GETTYKKTTS SALKGAIQLG ITHTVGSLST KPERDVLMQD FYVVESIFFP SEGSNLTPAH
121HYNDFRFKTY APVAFRYFRE LFGIRPDDYL YSLCSEPLIE LCSSGASGSL FYVSSDDEFI
181IKTVQHKEAE FLQKLLPGYY MNLNQNPRTL LPKFYGLYCV QAGGKNIRIV VMNNLLPRSV
241KMHIKYDLKG STYKRRASQK EREKPLPTFK DLDFLQDIPD GLFLDADMYN ALCKTLQRDC
301LVLQSFKIMD YSLLMSIHNI DHAQREPLSS ETQYSVDTRR PAPQKALYST AMESIQGEAR
361RGGTMETDDQ FVKKLEHSWK ALVHDGDTVS VHRPGFYAER FQRFMCNTVF KKIPLKPSPS
421KKFRSGSSFS RRAGSSGNSC ITYQPSVSGE HKAQVTTKAE VEPGVHLGRP DVLPQTPPLE
481EISEGSPIPD PSFSPLVGET LQMLTTSTTL EKLEVAESEF TH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

522 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.