PIP5K1A Antibody
Catalog Number: LS-C409488
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PIP5K1A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G81824-20
Catalog Number: LS-G81824
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PIP5K1 alpha(PIP5K1A) (NM_001135636) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PIP5K1A | PIP5KIalpha | PtdIns(4)P-5-kinase 1 alpha | PIP5K1-alpha
- Origin Species
- Human
- NCBI GenBank
- NM_003557
- SwissProt
- Q99755
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASASSGPSS SVGFSSFDPA VPSCTLSSAA SGIKRPMASE VPYASGMPIK KIGHRSVDSS61GETTYKKTTS SALKGAIQLG ITHTVGSLST KPERDVLMQD FYVVESIFFP SEGSNLTPAH121HYNDFRFKTY APVAFRYFRE LFGIRPDDYL YSLCSEPLIE LCSSGASGSL FYVSSDDEFI181IKTVQHKEAE FLQKLLPGYY MNLNQNPRTL LPKFYGLYCV QAGGKNIRIV VMNNLLPRSV241KMHIKYDLKG STYKRRASQK EREKPLPTFK DLDFLQDIPD GLFLDADMYN ALCKTLQRDC301LVLQSFKIMD YSLLMSIHNI DHAQREPLSS ETQYSVDTRR PAPQKALYST AMESIQGEAR361RGGTMETDDQ FVKKLEHSWK ALVHDGDTVS VHRPGFYAER FQRFMCNTVF KKIPLKPSPS421KKFRSGSSFS RRAGSSGNSC ITYQPSVSGE HKAQVTTKAE VEPGVHLGRP DVLPQTPPLE481EISEGSPIPD PSFSPLVGET LQMLTTSTTL EKLEVAESEF THTRTRPLEQKLISEEDLAANDILDYKDDDDKV
522 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
