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PHF14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHF14 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113784-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHF14 (NM_014660), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0783 | PHD finger protein 14 | PHF14
Origin Species
Human
NCBI GenBank
AC007029
SwissProt
O94880

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRSSKRRQV KPLAASLLEA LDYDSSDDSD FKVGDASDSE GSGNGSEDAS KDSGEGSCSD
61SEENILEEEL NEDIKVKEEQ LKNSAEEEVL SSEKQLIKME KKEEEENGER PRKKKEKEKE
121KEKEKEKEKE REKEKEKATV SENVAASAAA TTPATSPPAV NTSPSVPTTT TATEEQVSEP
181KKWNLRRNRP LLDFVSMEEL NDMDDYDSED DNDWRPTVVK RKGRSASQKE GSDGDNEDDE
241DEGSGSDEDE NDEGNDEDHS SPASEGGCKK KKSKVLSRNS ADDEELTNDS LTLSQSKSNE
301DSLILEKSQN WSSQKMDHIL ICCVCLGDNS EDADEIIQCD NCGITVHEGC YGVDGESDSI
361MSSASENSTE PWFCDACKCG VSPSCELCPN QDGIFKETDA GRWVHIVCAL YVPGVAFGDI
421DKLRPVTLTE MNYSKYGAKE CSFCEDPRFA RTGVCISCDA GMCRAYFHVT CAQKEGLLSE
481AAAEEDIADP FFAYCKQHAD RLDRKWKRKN YLALQSYCKM SLQEREKQLS PEAQARINAR
541LQQYRAKAEL ARSTRPQAWV PREKLPRPLT SSASAIRKLM RKAELMGIST DIFPVDNSDT
601SSSVDGRRKH KQPALTADFV NYYFERNMRM IQIQENMAEQ KNIKDKLENE QEKLHVEYNK
661LCESLEELQN LNGKLRSEGQ GIWALLGRIT GQKLNIPAIL RAPKERKPSK KEGGTQKTST
721LPAVLYSCGI CKKNHDQHLL LLCDTCKLHY HLGCLDPPLT RMPRKTKNSY WQCSECDQAG
781SSDMEADMAM ETLPDGTKRS RRQIKEPVKF VPQDVPPEPK KIPIRNTRTR GRKRSFVPEE
841EKHEERVPRE RRQRQSVLQK KPKAEDLRTE CATCKGTGDN ENLVRYPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

888 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.