PHF14 Antibody
Catalog Number: LS-C667641-100
100 µg / $359.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PHF14 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83442-20
Catalog Number: LS-G83442
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PHF14 (NM_014660) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0783 | PHD finger protein 14 | PHF14
- Origin Species
- Human
- NCBI GenBank
- AC007029
- SwissProt
- O94880
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRSSKRRQV KPLAASLLEA LDYDSSDDSD FKVGDASDSE GSGNGSEDAS KDSGEGSCSD61SEENILEEEL NEDIKVKEEQ LKNSAEEEVL SSEKQLIKME KKEEEENGER PRKKKEKEKE121KEKEKEKEKE REKEKEKATV SENVAASAAA TTPATSPPAV NTSPSVPTTT TATEEQVSEP181KKWNLRRNRP LLDFVSMEEL NDMDDYDSED DNDWRPTVVK RKGRSASQKE GSDGDNEDDE241DEGSGSDEDE NDEGNDEDHS SPASEGGCKK KKSKVLSRNS ADDEELTNDS LTLSQSKSNE301DSLILEKSQN WSSQKMDHIL ICCVCLGDNS EDADEIIQCD NCGITVHEGC YGVDGESDSI361MSSASENSTE PWFCDACKCG VSPSCELCPN QDGIFKETDA GRWVHIVCAL YVPGVAFGDI421DKLRPVTLTE MNYSKYGAKE CSFCEDPRFA RTGVCISCDA GMCRAYFHVT CAQKEGLLSE481AAAEEDIADP FFAYCKQHAD RLDRKWKRKN YLALQSYCKM SLQEREKQLS PEAQARINAR541LQQYRAKAEL ARSTRPQAWV PREKLPRPLT SSASAIRKLM RKAELMGIST DIFPVDNSDT601SSSVDGRRKH KQPALTADFV NYYFERNMRM IQIQENMAEQ KNIKDKLENE QEKLHVEYNK661LCESLEELQN LNGKLRSEGQ GIWALLGRIT GQKLNIPAIL RAPKERKPSK KEGGTQKTST721LPAVLYSCGI CKKNHDQHLL LLCDTCKLHY HLGCLDPPLT RMPRKTKNSY WQCSECDQAG781SSDMEADMAM ETLPDGTKRS RRQIKEPVKF VPQDVPPEPK KIPIRNTRTR GRKRSFVPEE841EKHEERVPRE RRQRQSVLQK KPKAEDLRTE CATCKGTGDN ENLVRYPSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
888 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
