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PHACTR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHACTR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHACTR2 antibodies →
$725.00each
Catalog Number: LS-G118878-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHACTR2 (NM_001100164), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf56 | PHACTR2 | KIAA0680
Origin Species
Human
NCBI GenBank
AB014580
SwissProt
O75167

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGQTSVSTLS PQPGSVDGLD KASIANSDGP TAGSQTPPFK RKGKLSTIGK IFKPWKWRKK
61KTSDKFRETS AVLERKISTR QSREELIRRG VLKELPDQDG DVTVNFENSN GHMIPIGEES
121TREENVVKSE EGNGSVSEKT PPLEEQAEDK KENTENHSET PAAPALPPSA PPKPRPKPKP
181KKSPVPPKGA TAGASHKGDE VPPIKKNTKA PGKQAPVPPP KPASRNTTRE AAGSSHSKKT
241TGSKASASPS TSSTSSRPKA SKETVSSKAG TVGTTKGKRK TDKQPITSHL SSDTTTSGTS
301DLKGEPAETR VESFKLEQTV PGAEEQNTGK FKSMVPPPPV APAPSPLAPP LPLEDQCITA
361SDTPVVLVSV GADLPVSALD PSQLLWAEEP TNRTTLYSGT GLSVNRENAK CFTTKEELGK
421TVPQLLTPGL MGESSESFSA SEDEGHREYQ ANDSDSDGPI LYTDDEDEDE DEDGSGESAL
481ASKIRRRDTL AIKLGNRPSK KELEDKNILQ RTSEEERQEI RQQIGTKLVR RLSQRPTTEE
541LEQRNILKQK NEEEEQEAKM ELKRRLSRKL SLRPTVAELQ ARRILRFNEY VEVTDSPDYD
601RRADKPWARL TPADKAAIRK ELNEFKSTEM EVHEESRQFT RFHRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

645 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.