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PHACTR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHACTR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113762-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHACTR2 (NM_014721), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf56 | PHACTR2 | KIAA0680
Origin Species
Human
NCBI GenBank
AB014580
SwissProt
O75167

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDNAVDGLDK ASIANSDGPT AGSQTPPFKR KGKLSTIGKI FKPWKWRKKK TSDKFRETSA
61VLERKISTRQ SREELIRRGV LKELPDQDGD VTVNFENSNG HMIPIGEEST REENVVKSEE
121GNGSVSEKTP PLEEQAEDKK ENTENHSETP AAPALPPSAP PKPRPKPKPK KSPVPPKGAT
181AGASHKGDEV PPIKKNTKAP GKQAPVPPPK PASRNTTREA AGSSHSKKTT GSKASASPST
241SSTSSRPKAS KETVSSKAGT VGTTKGKRKT DKQPITSHLS SDTTTSGTSD LKGEPAETRV
301ESFKLEQTVP GAEEQNTGKF KSMVPPPPVA PAPSPLAPPL PLEDQCITAS DTPVVLVSVG
361ADLPVSALDP SQLLWAEEPT NRTTLYSGTG LSVNRENAKC FTTKEELGKT VPQLLTPGLM
421GESSESFSAS EDEGHREYQA NDSDSDGPIL YTDDEDEDED EDGSGESALA SKIRRRDTLA
481IKLGNRPSKK ELEDKNILQR TSEEERQEIR QQIGTKLVRR LSQRPTTEEL EQRNILKQKN
541EEEEQEAKME LKRRLSRKLS LRPTVAELQA RRILRFNEYV EVTDSPDYDR RADKPWARLT
601PADKAAIRKE LNEFKSTEME VHEESRQFTR FHRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

634 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.