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PFKL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PFKL Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G122795-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PFKL (NM_001002021), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PFKL | Phosphofructokinase 1 | Phosphofructokinase | liver | PFK-B | Phosphohexokinase
Origin Species
Human
NCBI GenBank
NM_002626
SwissProt
P17858

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCNQGRGRES SRGGLHVQGS CRGLSRSPQQ ETGFAKAPAG TDCFFHCSPG SRGQGDRKEE
61VTSEPGGTSI MSRLGGMNAA VRAVTRMGIY VGAKVFLIYE GYEGLVEGGE NIKQANWLSV
121SNIIQLGGTI IGSARCKAFT TREGRRAAAY NLVQHGITNL CVIGGDGSLT GANIFRSEWG
181SLLEELVAEG KISETTARTY SHLNIAGLVG SIDNDFCGTD MTIGTDSALH RIMEVIDAIT
241TTAQSHQRTF VLEVMGRHCG YLALVSALAS GADWLFIPEA PPEDGWENFM CERLGETRSR
301GSRLNIIIIA EGAIDRNGKP ISSSYVKDLV VQRLGFDTRV TVLGHVQRGG TPSAFDRILS
361SKMGMEAVMA LLEATPDTPA CVVTLSGNQS VRLPLMECVQ MTKEVQKAMD DKRFDEATQL
421RGGSFENNWN IYKLLAHQKP PKEKSNFSLA ILNVGAPAAG MNAAVRSAVR TGISHGHTVY
481VVHDGFEGLA KGQVQEVGWH DVAGWLGRGG SMLGTKRTLP KGQLESIVEN IRIYGIHALL
541VVGGFEAYEG VLQLVEARGR YEELCIVMCV IPATISNNVP GTDFSLGSDT AVNAAMESCD
601RIKQSASGTK RRVFIVETMG GYCGYLATVT GIAVGADAAY VFEDPFNIHD LKVNVEHMTE
661KMKTDIQRGL VLRNEKCHDY YTTEFLYNLY SSEGKGVFDC RTNVLGHLQQ GGAPTPFDRN
721YGTKLGVKAM LWLSEKLREV YRKGRVFANA PDSACVIGLK KKAVAFSPVT ELKKDTDFEH
781RMPREQWWLS LRLMLKMLAQ YRISMAAYVS GELEHVTRRT LSMDKGF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

827 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.