PFKL Antibody
Catalog Number: LS-C409260
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PFKL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G74163-20
Catalog Number: LS-G74163
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PFKL (NM_001002021) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PFKL | Phosphofructokinase 1 | Phosphofructokinase | liver | PFK-B | Phosphohexokinase
- Origin Species
- Human
- NCBI GenBank
- NM_002626
- SwissProt
- P17858
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCNQGRGRES SRGGLHVQGS CRGLSRSPQQ ETGFAKAPAG TDCFFHCSPG SRGQGDRKEE61VTSEPGGTSI MSRLGGMNAA VRAVTRMGIY VGAKVFLIYE GYEGLVEGGE NIKQANWLSV121SNIIQLGGTI IGSARCKAFT TREGRRAAAY NLVQHGITNL CVIGGDGSLT GANIFRSEWG181SLLEELVAEG KISETTARTY SHLNIAGLVG SIDNDFCGTD MTIGTDSALH RIMEVIDAIT241TTAQSHQRTF VLEVMGRHCG YLALVSALAS GADWLFIPEA PPEDGWENFM CERLGETRSR301GSRLNIIIIA EGAIDRNGKP ISSSYVKDLV VQRLGFDTRV TVLGHVQRGG TPSAFDRILS361SKMGMEAVMA LLEATPDTPA CVVTLSGNQS VRLPLMECVQ MTKEVQKAMD DKRFDEATQL421RGGSFENNWN IYKLLAHQKP PKEKSNFSLA ILNVGAPAAG MNAAVRSAVR TGISHGHTVY481VVHDGFEGLA KGQVQEVGWH DVAGWLGRGG SMLGTKRTLP KGQLESIVEN IRIYGIHALL541VVGGFEAYEG VLQLVEARGR YEELCIVMCV IPATISNNVP GTDFSLGSDT AVNAAMESCD601RIKQSASGTK RRVFIVETMG GYCGYLATVT GIAVGADAAY VFEDPFNIHD LKVNVEHMTE661KMKTDIQRGL VLRNEKCHDY YTTEFLYNLY SSEGKGVFDC RTNVLGHLQQ GGAPTPFDRN721YGTKLGVKAM LWLSEKLREV YRKGRVFANA PDSACVIGLK KKAVAFSPVT ELKKDTDFEH781RMPREQWWLS LRLMLKMLAQ YRISMAAYVS GELEHVTRRT LSMDKGFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
827 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
