Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PEPD / PROLIDASE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128149-100
Catalog Number: LS-G128149
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PEPD (NM_001166056), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Aminoacyl-L-proline hydrolase | Imidodipeptidase | PEPD | PRD | PROLIDASE | X-Pro dipeptidase | Xaa-Pro dipeptidase | Peptidase D | Proline dipeptidase
- Origin Species
- Human
- NCBI GenBank
- NM_000285
- SwissProt
- P12955
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAATGPSFW LGNETLKVPL ALFALNRQRL CERLRKNPAV QAGSIVVLQG GEETQRYCTD61TGVLFRQESF FHWAFGVTEP GCYGVIDVDT GKSTLFVPRL PASHATWMGK IHSKEHFKEK121YAVDDVQYVD EIASVLTSQK PSVLLTLRGV NTDSGSVCRE ASFDGISKFE VNNTILHPEI181VECLFEHYCY SRGGMRHSSY TCICGSGENS AVLHYGHAGA PNDRTIQNGD MCLFDMGGEY241YCFASDITCS FPANGKFTAD QKAVYEAVLR SSRAVMGAMK PGVWWPDMHR LADRIHLEEL301AHMGILSGSV DAMVQAHLGA VFMPHGLGHF LGIDVHDVGG YPEGVERIDE PGLRSLRTAR361HLQPGMVLTV EPGIYFIDHL LDEALADPAR ASFLNREVLQ RFRGFGGVRI EEDVVVTDSG421IELLTCVPRT VEEIEACMAG CDKAFTPFSG PKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
452 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.