Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PEPD / PROLIDASE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128128-100
Catalog Number: LS-G128128
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PEPD (NM_001166057), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Aminoacyl-L-proline hydrolase | Imidodipeptidase | PEPD | PRD | PROLIDASE | X-Pro dipeptidase | Xaa-Pro dipeptidase | Peptidase D | Proline dipeptidase
- Origin Species
- Human
- NCBI GenBank
- NM_000285
- SwissProt
- P12955
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAATGPSFW LGNETLKVPL ALFALNRQRL CERLRKNPAV QAGSIVVLQG GEETQRYCTD61TGVLFRQIAS VLTSQKPSVL LTLRGVNTDS GSVCREASFD GISKFEVNNT ILHPEIVECR121VFKTDMELEV LRYTNKISSE AHREVMKAVK VGMKEYELES LFEHYCYSRG GMRHSSYTCI181CGSGENSAVL HYGHAGAPND RTIQNGDMCL FDMGGEYYCF ASDITCSFPA NGKFTADQKA241VYEAVLRSSR AVMGAMKPGV WWPDMHRLAD RIHLEELAHM GILSGSVDAM VQAHLGAVFM301PHGLGHFLGI DVHDVGGYPE GVERIDEPGL RSLRTARHLQ PGMVLTVEPG IYFIDHLLDE361ALADPARASF LNREVLQRFR GFGGVRIEED VVVTDSGIEL LTCVPRTVEE IEACMAGCDK421AFTPFSGPKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
429 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.