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PEG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PEG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PEG3 antibodies →
$725.00each
Catalog Number: LS-G128465-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PEG3 (NM_001146184), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0287 | Paternally expressed 3 | PEG3 | PW1 | ZNF904 | ZKSCAN22 | ZSCAN24
Origin Species
Human
NCBI GenBank
NM_006210
SwissProt
Q9GZU2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
180.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLPPKHLSAT KPKKSWAPNL YELDSDLTKE PDVIIGEGPT DSEFFHQRFR NLIYVEFVGP
61RKTLIKLRNL CLDWLQPETR TKEEIIELLV LEQYLTIIPE KLKPWVRAKK PENCEKLVTL
121LENYKEMYQP EDDNNSDVTS DDDMTRNRRE SSPPHSVHSF SGDRDWDRRG RSRDMEPRDR
181WSHTRNPRSR MPPRDLSLPV VAKTSFEMDR EDDRDSRAYE SRSQDAESYQ NVVDLAEDRK
241PHNTIQDNME NYRKLLSLGV QLAEDDGHSH MTQGHSSRSK RSAYPSTSRG LKTMPEAKKS
301THRRGICEDE SSHGVIMEKF IKDVSRSSKS GRARESSDRS QRFPRMSDDN WKDISLNKRE
361SVIQQRVYEG NAFRGGFRFN STLVSRKRVL ERKRRYHFDT DGKGSIHDQK GCPRKKPFEC
421GSEMRKAMSV SSLSSLSSPS FTESQPIDFG AMPYVCDECG RSFSVISEFV EHQIMHTREN
481LYEYGESFIH SVAVSEVQKS QVGGKRFECK DCGETFNKSA ALAEHRKIHA RGYLVECKNQ
541ECEEAFMPSP TFSELQKIYG KDKFYECRVC KETFLHSSAL IEHQKIHFGD DKDNEREHER
601ERERERGETF RPSPALNEFQ KMYGKEKMYE CKVCGETFLH SSSLKEHQKI HTRGNPFENK
661GKVCEETFIP GQSLKRRQKT YNKEKLCDFT DGRDAFMQSS ELSEHQKIHS RKNLFEGRGY
721EKSVIHSGPF TESQKSHTIT RPLESDEDEK AFTISSNPYE NQKIPTKENV YEAKSYERSV
781IHSLASVEAQ KSHSVAGPSK PKVMAESTIQ SFDAINHQRV RAGGNTSEGR EYSRSVIHSL
841VASKPPRSHN GNELVESNEK GESSIYISDL NDKRQKIPAR ENPCEGGSKN RNYEDSVIQS
901VFRAKPQKSV PGEGSGEFKK DGEFSVPSSN VREYQKARAK KKYIEHRSNE TSVIHSLPFG
961EQTFRPRGML YECQECGECF AHSSDLTEHQ KIHDREKPSG SRNYEWSVIR SLAPTDPQTS
1021YAQEQYAKEQ ARNKCKDFRQ FFATSEDLNT NQKIYDQEKS HGEESQGENT DGEETHSEET
1081HGQETIEDPV IQGSDMEDPQ KDDPDDKIYE CEDCGLGFVD LTDLTDHQKV HSRKCLVDSR
1141EYTHSVIHTH SISEYQRDYT GEQLYECPKC GESFIHSSFL FEHQRIHEQD QLYSMKGCDD
1201GFIALLPMKP RRNRAAERNP ALAGSAIRCL LCGQGFIHSS ALNEHMRLHR EDDLLEQSQM
1261AEEAIIPGLA LTEFQRSQTE ERLFECAVCG ESFVNPAELA DHVTVHKNEP YEYGSSYTHT
1321SFLTEPLKGA IPFYECKDCG KSFIHSTVLT KHKELHLEEE EEDEAAAAAA AAAQEVEANV
1381HVPQVVLRIQ GLNVEAAEPE VEAAEPEVEA AEPEVEAAEP NGEAEGPDGE AAEPIGEAGQ
1441PNGEAEQPNG DADEPDGAGI EDPEERAEEP EGKAEEPEGD ADEPDGVGIE DPEEGEDQEI
1501QVEEPYYDCH ECTETFTSST AFSEHLKTHA SMIIFEPANA FGECSGYIER ASTSTGGANQ
1561ADEKYFKCDV CGQLFNDRLS LARHQNTHTG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1590 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.