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PEG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PEG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PEG3 antibodies →
$725.00each
Catalog Number: LS-G100762-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PEG3 (NM_006210), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0287 | Paternally expressed 3 | PEG3 | PW1 | ZNF904 | ZKSCAN22 | ZSCAN24
Origin Species
Human
NCBI GenBank
NM_006210
SwissProt
Q9GZU2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
180.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLPPKHLSAT KPKKSWAPNL YELDSDLTKE PDVIIGEGPT DSEFFHQRFR NLIYVEFVGP
61RKTLIKLRNL CLDWLQPETR TKEEIIELLV LEQYLTIIPE KLKPWVRAKK PENCEKLVTL
121LENYKEMYQP EDDNNSDVTS DDDMTRNRRE SSPPHSVHSF SDRDWDRRGR SRDMEPRDRW
181SHTRNPRSRM PPRDLSLPVV AKTSFEMDRE DDRDSRAYES RSQDAESYQN VVDLAEDRKP
241HNTIQDNMEN YRKLLSLVQL AEDDGHSHMT QGHSSRSKRS AYPSTSRGLK TMPEAKKSTH
301RRGICEDESS HGVIMEKFIK DVSRSSKSGR ARESSDRSQR FPRMSDDNWK DISLNKRESV
361IQQRVYEGNA FRGGFRFNST LVSRKRVLER KRRYHFDTDG KGSIHDQKGC PRKKPFECGS
421EMRKAMSVSS LSSLSSPSFT ESQPIDFGAM PYVCDECGRS FSVISEFVEH QIMHTRENLY
481EYGESFIHSV AVSEVQKSQV GGKRFECKDC GETFNKSAAL AEHRKIHARG YLVECKNQEC
541EEAFMPSPTF SELQKIYGKD KFYECRVCKE TFLHSSALIE HQKIHFGDDK DNEREHERER
601ERERGETFRP SPALNEFQKM YGKEKMYECK VCGETFLHSS SLKEHQKIHT RGNPFENKGK
661VCEETFIPGQ SLKRRQKTYN KEKLCDFTDG RDAFMQSSEL SEHQKIHSRK NLFEGRGYEK
721SVIHSGPFTE SQKSHTITRP LESDEDEKAF TISSNPYENQ KIPTKENVYE AKSYERSVIH
781SLASVEAQKS HSVAGPSKPK VMAESTIQSF DAINHQRVRA GGNTSEGREY SRSVIHSLVA
841SKPPRSHNGN ELVESNEKGE SSIYISDLND KRQKIPAREN PCEGGSKNRN YEDSVIQSVF
901RAKPQKSVPG EGSGEFKKDG EFSVPSSNVR EYQKARAKKK YIEHRSNETS VIHSLPFGEQ
961TFRPRGMLYE CQECGECFAH SSDLTEHQKI HDREKPSGSR NYEWSVIRSL APTDPQTSYA
1021QEQYAKEQAR NKCKDFRQFF ATSEDLNTNQ KIYDQEKSHG EESQGENTDG EETHSEETHG
1081QETIEDPVIQ GSDMEDPQKD DPDDKIYECE DCGLGFVDLT DLTDHQKVHS RKCLVDSREY
1141THSVIHTHSI SEYQRDYTGE QLYECPKCGE SFIHSSFLFE HQRIHEQDQL YSMKGCDDGF
1201IALLPMKPRR NRAAERNPAL AGSAIRCLLC GQGFIHSSAL NEHMRLHRED DLLEQSQMAE
1261EAIIPGLALT EFQRSQTEER LFECAVCGES FVNPAELADH VTVHKNEPYE YGSSYTHTSF
1321LTEPLKGAIP FYECKDCGKS FIHSTVLTKH KELHLEEEEE DEAAAAAAAA AQEVEANVHV
1381PQVVLRIQGL NVEAAEPEVE AAEPEVEAAE PEVEAAEPNG EAEGPDGEAA EPIGEAGQPN
1441GEAEQPNGDA DEPDGAGIED PEERAEEPEG KAEEPEGDAD EPDGVGIEDP EEGEDQEIQV
1501EEPYYDCHEC TETFTSSTAF SEHLKTHASM IIFEPANAFG ECSGYIERAS TSTGGANQAD
1561EKYFKCDVCG QLFNDRLSLA RHQNTHTG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1588 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.