Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PDLIM5 / LIM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121770-100
Catalog Number: LS-G121770
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDLIM5 (NM_001011513), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ENH1 | L9 | PDLIM5 | PDZ and LIM domain protein 5 | Lim | PDZ and LIM domain 5 | ENH | Enigma homolog | Enigma-like LIM domain protein
- Origin Species
- Human
- NCBI GenBank
- NM_006457
- SwissProt
- Q96HC4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSNYSVSLVG PAPWGFRLQG GKDFNMPLTI SSLKDGGKAA QANVRIGDVV LSIDGINAQG61MTHLEAQNKI KGCTGSLNMT LQRASAAPKP EPVPVQKPTV TSVCSETSQE LAEGQRRGSQ121GDSKQQNGPP RKHIVERYTE FYHVPTHSDA SKKRLIEDTE DWRPRTGTTQ SRSFRILAQI181TGTEHLKESE ADNTKKANNS QEPSPQLASS VASTRSMPES LDSPTSGRPG VTSLTTAAAF241KPVGSTGVIK SPSWQRPNQG VPSTGRISNS ATYSGSVAPA NSALGQTQPS DQDTLVQRAE301HIPAGKRTPM CAHCNQVIRG PFLVALGKSW HPEEFNCAHC KNTMAYIGFV EEKGALYCEL361CYEKFFAPEC GRCQRKILGE VISALKQTWH VSCFVCVACG KPIRNNVFHL EDGEPYCETD421YYALFGTICH GCEFPIEAGD MFLEALGYTW HDTCFVCSVC CESLEGQTFF SKKDKPLCKK481HAHSVNFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
487 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.