PDLIM5 / LIM Antibody
Catalog Number: LS-C753361-100
100 µg / $338.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PDLIM5 / LIM Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G82956-20
Catalog Number: LS-G82956
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PDLIM5 (NM_001011513) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ENH1 | L9 | PDLIM5 | PDZ and LIM domain protein 5 | Lim | PDZ and LIM domain 5 | ENH | Enigma homolog | Enigma-like LIM domain protein
- Origin Species
- Human
- NCBI GenBank
- NM_006457
- SwissProt
- Q96HC4
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSNYSVSLVG PAPWGFRLQG GKDFNMPLTI SSLKDGGKAA QANVRIGDVV LSIDGINAQG61MTHLEAQNKI KGCTGSLNMT LQRASAAPKP EPVPVQKPTV TSVCSETSQE LAEGQRRGSQ121GDSKQQNGPP RKHIVERYTE FYHVPTHSDA SKKRLIEDTE DWRPRTGTTQ SRSFRILAQI181TGTEHLKESE ADNTKKANNS QEPSPQLASS VASTRSMPES LDSPTSGRPG VTSLTTAAAF241KPVGSTGVIK SPSWQRPNQG VPSTGRISNS ATYSGSVAPA NSALGQTQPS DQDTLVQRAE301HIPAGKRTPM CAHCNQVIRG PFLVALGKSW HPEEFNCAHC KNTMAYIGFV EEKGALYCEL361CYEKFFAPEC GRCQRKILGE VISALKQTWH VSCFVCVACG KPIRNNVFHL EDGEPYCETD421YYALFGTICH GCEFPIEAGD MFLEALGYTW HDTCFVCSVC CESLEGQTFF SKKDKPLCKK481HAHSVNFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
487 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
