Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PDHA1 / PDH E1 Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129733-100
Catalog Number: LS-G129733
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDHA1 (NM_001173455), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PDHCE1A | PDHA | PHE1A | PDHA1 | PDHE1-A type I
- Origin Species
- Human
- NCBI GenBank
- NM_000284
- SwissProt
- P08559
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRKMLAAVSR VLSGASQKPA SRVLVASRNF ANDATFEIKK CDLHRLEEGP PVTTVLTRED61GLKYYRMMQT VRRMELKADQ LYKQKIIRGF CHLCDGQFLL PLTQEACCVG LEAGINPTDH121LITAYRAHGF TFTRGLSVRE ILAELTGRKG GCAKGKGGSM HMYAKNFYGG NGIVGAQVPL181GAGIALACKY NGKDEVCLTL YGDGAANQGQ IFEAYNMAAL WKLPCIFICE NNRYGMGTSV241ERAAASTDYY KRGDFIPGLR VDGMDILCVR EATRFAAAYC RSGKGPILME LQTYRYHGHS301MSDPGVSYRT REEIQEVRSK SDPIMLLKDR MVNSNLASVE ELKEIDVEVR KEIEDAAQFA361TADPEPPLEE LGYHIYSSDP PFEVRGANQW IKFKSVSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
397 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.