PDHA1 / PDH E1 Alpha Antibody
Catalog Number: LS-C331751
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PDHA1 / PDH E1 Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G78497-20
Catalog Number: LS-G78497
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PDHA1 (NM_001173454) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PDHCE1A | PDHA | PHE1A | PDHA1 | PDHE1-A type I
- Origin Species
- Human
- NCBI GenBank
- NM_000284
- SwissProt
- P08559
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRKMLAAVSR VLSGASQKPR HGLATLPSLV SISRLKQSSH LGLPKCWDYS HSLKTRQASR61VLVASRNFAN DATFEIKKCD LHRLEEGPPV TTVLTREDGL KYYRMMQTVR RMELKADQLY121KQKIIRGFCH LCDGQEACCV GLEAGINPTD HLITAYRAHG FTFTRGLSVR EILAELTGRK181GGCAKGKGGS MHMYAKNFYG GNGIVGAQVP LGAGIALACK YNGKDEVCLT LYGDGAANQG241QIFEAYNMAA LWKLPCIFIC ENNRYGMGTS VERAAASTDY YKRGDFIPGL RVDGMDILCV301REATRFAAAY CRSGKGPILM ELQTYRYHGH SMSDPGVSYR TREEIQEVRS KSDPIMLLKD361RMVNSNLASV EELKEIDVEV RKEIEDAAQF ATADPEPPLE ELGYHIYSSD PPFEVRGANQ421WIKFKSVSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
428 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
