LSBio
PDE4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PDE4B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PDE4B antibodies →
$725.00each
Catalog Number: LS-G99304-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PDE4(PDE4B) (NM_001037339), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Pde-ivb | PDE32 | PDE4B5 | PDEIVB | Phosphodiesterase 4b | DPDE4 | PDE4B | PDE4B3
NCBI GenBank
NM_002600
SwissProt
Q07343

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKEHGGTFSS TGISGGSGDS AMDSLQPLQP NYMPVCLFAE ESYQKLAMET LEELDWCLDQ
61LETIQTYRSV SEMASNKFKR MLNRELTHLS EMSRSGNQVS EYISNTFLDK QNDVEIPSPT
121QKDREKKKKQ QLMTQISGVK KLMHSSSLNN TSISRFGVNT ENEDHLAKEL EDLNKWGLNI
181FNVAGYSHNR PLTCIMYAIF QERDLLKTFR ISSDTFITYM MTLEDHYHSD VAYHNSLHAA
241DVAQSTHVLL STPALDAVFT DLEILAAIFA AAIHDVDHPG VSNQFLINTN SELALMYNDE
301SVLENHHLAV GFKLLQEEHC DIFMNLTKKQ RQTLRKMVID MVLATDMSKH MSLLADLKTM
361VETKKVTSSG VLLLDNYTDR IQVLRNMVHC ADLSNPTKSL ELYRQWTDRI MEEFFQQGDK
421ERERGMEISP MCDKHTASVE KSQVGFIDYI VHPLWETWAD LVQPDAQDIL DTLEDNRNWY
481QSMIPQSPSP PLDEQNRDCQ GLMEKFQFEL TLDEEDSEGP EKEGEGHSYF SSTKTLCVID
541PENRDSLGET DIDIATEDKS PVDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

564 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.