PDE4B Antibody
Catalog Number: LS-C496904
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human PDE4B Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G74954-20
Catalog Number: LS-G74954
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PDE4(PDE4B) (NM_001037339) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Pde-ivb | PDE32 | PDE4B5 | PDEIVB | Phosphodiesterase 4b | DPDE4 | PDE4B | PDE4B3
- Origin Species
- Human
- NCBI GenBank
- NM_002600
- SwissProt
- Q07343
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKEHGGTFSS TGISGGSGDS AMDSLQPLQP NYMPVCLFAE ESYQKLAMET LEELDWCLDQ61LETIQTYRSV SEMASNKFKR MLNRELTHLS EMSRSGNQVS EYISNTFLDK QNDVEIPSPT121QKDREKKKKQ QLMTQISGVK KLMHSSSLNN TSISRFGVNT ENEDHLAKEL EDLNKWGLNI181FNVAGYSHNR PLTCIMYAIF QERDLLKTFR ISSDTFITYM MTLEDHYHSD VAYHNSLHAA241DVAQSTHVLL STPALDAVFT DLEILAAIFA AAIHDVDHPG VSNQFLINTN SELALMYNDE301SVLENHHLAV GFKLLQEEHC DIFMNLTKKQ RQTLRKMVID MVLATDMSKH MSLLADLKTM361VETKKVTSSG VLLLDNYTDR IQVLRNMVHC ADLSNPTKSL ELYRQWTDRI MEEFFQQGDK421ERERGMEISP MCDKHTASVE KSQVGFIDYI VHPLWETWAD LVQPDAQDIL DTLEDNRNWY481QSMIPQSPSP PLDEQNRDCQ GLMEKFQFEL TLDEEDSEGP EKEGEGHSYF SSTKTLCVID541PENRDSLGET DIDIATEDKS PVDTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
564 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
