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PCF11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PCF11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PCF11 antibodies →
$504.00each
Catalog Number: LS-G113010-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PCF11 (NM_015885), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PCF11 | PCF11p homolog | KIAA0824
Origin Species
Human
NCBI GenBank
NM_015885
SwissProt
O94913

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
173.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEQTPAEAG AAGAREDACR DYQSSLEDLT FNSKPHINML TILAEENLPF AKEIVSLIEA
61QTAKAPSSEK LPVMYLMDSI VKNVGREYLT AFTKNLVATF ICVFEKVDEN TRKSLFKLRS
121TWDEIFPLKK LYALDVRVNS LDPAWPIKPL PPNVNTSSIH VNPKFLNKSP EEPSTPGTVV
181SSPSISTPPI VPDIQKNLTQ EQLIRQQLLA KQKQLLELQQ KKLELELEQA KAQLAVSLSV
241QQETSNLGPG SAPSKLHVSQ IPPMAVKAPH QVPVQSEKSR PGPSLQIQDL KGTNRDPRLN
301RISQHSHGKD QSHRKEFLMN TLNQSDTKTS KTIPSEKLNS SKQEKSKSGE KITKKELDQL
361DSKSKSKSKS PSPLKNKLSH TKDLKNQESE SMRLSDMNKR DPRLKKHLQD KTDGKDDDVK
421EKRKTAEKKD KDEHMKSSEH RLAGSRNKII NGIVQKQDTI TEESEKQGTK PGRSSTRKRS
481RSRSPKSRSP IIHSPKRRDR RSPKRRQRSM SPTSTPKAGK IRQSGAKQSH MEEFTPPSRE
541DRNAKRSTKQ DIRDPRRMKK TEEERPQETT NQHSTKSGTE PKENVENWQS SKSAKRWKSG
601WEENKSLQQV DEHSKPPHLR HRESWSSTKG ILSPRAPKQQ QHRLSVDANL QIPKELTLAS
661KRELLQKTSE RLASGEITQD DFLVVVHQIR QLFQYQEGVR EEQRSPFNDR FPLKRPRYED
721SDKPFVDSPA SRFAGLDTNQ RLTALAEDRP LFDGPSRPSV ARDGPTKMIF EGPNKLSPRI
781DGPPTPASLR FDGSPGQMGG GGPLRFEGPQ GQLGGGCPLR FEGPPGPVGT PLRFEGPIGQ
841AGGGGFRFEG SPGLRFEGSP GGLRFEGPGG QPVGGLRFEG HRGQPVGGLR FEGPHGQPVG
901GLRFDNPRGQ PVGGLRFEGG HGPSGAAIRF DGPHGQPGGG IRFEGPLLQQ GVGMRFEGPH
961GQSVAGLRFE GQHNQLGGNL RFEGPHGQPG VGIRFEGPLV QQGGGMRFEG PSVPGGGLRI
1021EGPLGQGGPR FEGCHALRFD GQPGQPSLLP RFDGLHGQPG PRFERTPGQP GPQRFDGPPG
1081QQVQPRFDGV PQRFDGPQHQ QASRFDIPLG LQGTRFDNHP SQRLESVSFN QTGPYNDPPG
1141NAFNAPSQGL QFQRHEQIFD SPQGPNFNGP HGPGNQSFSN PLNRASGHYF DEKNLQSSQF
1201GNFGNIPAPM TVGNIQASQQ VLSGVAQPVA FGQGQQFLPV HPQNPGFVQN PSGALPKAYP
1261DNHLSQVDVN ELFSKLLKTG ILKLSQTDSA TTQVSEVTAQ PPPEEEEDQN EDQDVPDLTN
1321FTVEELKQRY DSVINRLYTG IQCYSCGMRF TTSQTDVYAD HLDWHYRQNR TEKDVSRKVT
1381HRRWYYSLTD WIEFEEIADL EERAKSQFFE KVHEEVVLKT QEAAKEKEFQ SVPAGPAGAV
1441ESCEICQEQF EQYWDEEEEE WHLKNAIRVD GKIYHPSCYE DYQNTSSFDC TPSPSKTPVE
1501NPLNIMLNIV KNELQEPCDS PKVKEERIDT PPACTEESIA TPSEIKTEND TVESV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1555 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.